Journal of the Indian Academy of Wood Science

, Volume 15, Issue 2, pp 115–119 | Cite as

Effect of wood, bark and leaf extracts of Macaranga trees on cytotoxic activity in some cancer and normal cell lines

  • Enos Tangke ArungEmail author
  • Rudianto Amirta
  • Qinchang Zhu
  • Yhiya Amen
  • Kuniyoshi ShimizuEmail author
Original Article


The genus of Macaranga (Euphorbiacaceae) has 250 species of which 160 species are endemic in Kalimantan and New Guinea. They grow as pioneer trees and are used as traditional medicines in the Asian regions. This experiment concerns cytotoxicity in both cancer and normal cells of their methanol extracts from wood, bark and leaf parts. Some of them have not yet reported its cytotoxic activity in those cell lines. The 21 methanol extracts were prepared from seven Macaranga tree species (Macaranga bancana, Macaranga gigantea, Macaranga hullettii, Macaranga pruinosa, Macaranga tanarius, Macaranga trichocarpa and Macaranga triloba). The MTT assay was used to evaluate cytotoxic activity of extracts in cancer cell lines [human breast cancer (MCF-7), mouse melanoma (B16 melanoma), human colon cancer (HCT116), human cervical adenocarcinoma (HeLa)] and normal cell lines [human normal fibroblast (TIG-1) and normal human dermal fibroblast (NHDF)]. The leaf extracts of M. pruinosa, M. tanarius, M. trichocarpa showed more cytotoxicity than wood and bark in all cancer cell lines. In addition, the 5-fluorouracil was used as a positive control. These findings indicated the extracts from leaves of M. pruinosa, M. tanarius and M. trichocarpa had more potential than wood and bark parts to be developed as anti-cancer agents.


Macaranga trees Cytotoxic activity Cancer cells Normal cells 



A part of this work was financially supported by Islamic Development Bank (IDB) Project for Mulawarman University 2018 (No. 2248/UN17.11/PL/2018) and the Grant of Mulawarman University Research of Excellent Program (UNMUL PUPT—Grant Nos. 166/UN17.16/PG/2015 and 104/UN17.41/LT/2016) provided by the Directorate General of Higher Education, the Ministry of Research, Technology, and Higher Education of Indonesia (Kemenristekdikti).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Indian Academy of Wood Science 2018

Authors and Affiliations

  1. 1.Laboratory of Forest Product Chemistry, Faculty of ForestryMulawarman UniversitySamarindaIndonesia
  2. 2.Laboratory of Industrial Biotechnology, Faculty of ForestryMulawarman UniversitySamarindaIndonesia
  3. 3.Department of Agro-Environmental Sciences, Faculty of AgricultureKyushu UniversityFukuokaJapan
  4. 4.Department of Pharmacognosy, Faculty of PharmacyMansoura UniversityMansouraEgypt
  5. 5.Department of Pharmacy, School of MedicineShenzhen UniversityShenzhenChina

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