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Intramammary honey preparation for treatment of subclinical bovine mastitis: a preliminary study

  • Mokhtar BenhanifiaEmail author
  • Abdelhanine Ayad
  • Wessam Mohamed
Article
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Abstract

Honey has been used in medicine against bacterial infections for thousands of years. The objective of this study was to evaluate the efficacy of intramammary honey administered during lactating period to eliminate Staphylococcus aureus and Pseudomonas aeruginosa infection. Three infected cows were investigated. Five milliliters of multifloral undiluted honey was administered aseptically in quarter by intramammary infusion during two consecutive days (day 1 and day 2) of experiment. Cows’ milk samples were collected for bacteriological analysis during interval of time: 0, 7, 14, 21, 52, and 60 days. The cure following administration of honey was two cows at 7 and 14 days; three at 21, 52, and 60 days for S. aureus infection and two cows at days 21, 52, and 60 for P. aeruginosa. In this preliminary study, the efficacy of honey has demonstrated as one of the new alternative therapeutic product against P. aeruginosa and S. aureus subclinical mastitis. Further studies may elucidate the susceptibility of pathogens microorganisms implicated in acute clinical and subclinical bovine mastitis, the dynamic of honey in mammary gland, and appropriate intramammary infusion.

Keywords

Subclinical mastitis Honey Bovine In vivo 
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Notes

References

  1. Abdul Hafeez MM, Ali MM, Abdel-Rahman MF, Nahed Wahba M (2005) Antibacterial activity of honey for treatment of subclinical mastitis: 2-intramammary infusion as a tool to manage non responding antibiotic cases. 8th scientific congress of cattle disease Egypt, 146–14Google Scholar
  2. Acar JF, Moulin G (2006) Antimicrobial resistance at farm level. Revue scientifique et technique - Office International des eEpizooties 25:775–792Google Scholar
  3. Alandejani T, Marsan J, Ferris W, Slinger R, Chan F (2009) Effectiveness of honey on Staphylococcus aureus and Pseudomonas aeruginosa biofilms. Otolaryngol Head Neck Surg 141(1):114–118Google Scholar
  4. Allen KL, Molan PC (1997) The sensitivity of mastitis-causing bacteria to the antibacterial activity of honey. N Z J Agric Res 40(4):537–540Google Scholar
  5. Al-Waili N, Al-Ghamdi A, Ansari MJ, Al-Attalm Y, Salom K (2012) Synergistic effects of honey and propolis toward drug multi-resistant and Candida albicans isolates in single and polymicrobial cultures. Int J Med Sci 9(9):793–800Google Scholar
  6. Al-Waili NS, Al-Waili FS, Akmal M, Ali A, Salom KY, Al Ghamdi AA (2014) Effects of natural honey on polymicrobial culture of various human pathogens. Arch Med Sci 10(2):246–250Google Scholar
  7. Bačić G, Mačešić N, Radin L, Aladrović J, Matanović K, Mašek T, Brozić D, Benić M, Radić B, Bačić I, Šuran J (2016) Intramammary propolis formulation for prevention and treatment of mastitis in dairy ruminants (RC.2.2.08-0003). J Anim Res 6(2):47–49Google Scholar
  8. Barkema HW, Schukken YH, Lam TJ, Beiboer ML, Wilmink H, Benedictus G, Brand A (1998) Incidence of clinical mastitis in dairy herds grouped in three categories by bulk milk somatic cell counts. J Dairy Sci 81(2):411–419Google Scholar
  9. Barkema HW, Green MJ, Bradley AJ, Zadoks RN (2009) The role of contagious disease in udder health. J Dairy Sci 92(10):4717–4729Google Scholar
  10. Benhamed N, Kihal M (2013) Phenotypic and genotypic characterization of Staphylococcus aureus agents of dairy cows’ mastitis in Algeria. J Appl Sci Res 9:86–93Google Scholar
  11. Benhanifia MB, Boukraâ L, Hammoudi SM, Sulaiman SA, Manivannan L (2011) Recent patents on topical application of honey in wound and burn management. Recent Pat Inflamm Allergy Drug Discov 5(1):81–86Google Scholar
  12. Boufaida Asnoune Z, Butel MJ, Ouzrout R (2012) Prévalence des principales bactéries responsables de mammites subcliniques des vaches laitières au nord-est de l’Algérie. Rev Elev Méd Vét Pays Trop 655–9Google Scholar
  13. Boukraâ L, Niar A, Benbarek H, Benhanifia M (2008) Additive action of royal jelly and honey against Staphylococcus aureus. J Med Food 11(1):190–192Google Scholar
  14. Bourabah A, Ayad A, Hammoudi SM, Boukraa L, Benbarek H (2014) Antimicrobial activity of Algerian honey on subclinical mastitis pathogens isolated from goat’s milk. Vet World 7(4):248–252Google Scholar
  15. Bradley A (2002) Bovine mastitis: an evolving disease. Vet J 164(2):116–128Google Scholar
  16. CDC, Centers for Disease Control and Prevention (2012) About antimicrobial resistance: a brief overview. http://www.cdc.gov/drugresistance/about.html
  17. Cianciosi D, Forbes-Hernández TY, Afrin S, Gasparrini M, Reboredo-Rodriguez P, Manna PP, Zhang J, Bravo Lamas L, Martínez Flórez S, Agudo Toyos P, Quiles JL, Giampieri F, Battino M (2018) Phenolic compounds in honey and their associated health benefits: a review. Molecules 23(9):2322Google Scholar
  18. Coats LM (1998) An outbreak of Pseudomonas mastitis. NZ Vet J 46(1):39Google Scholar
  19. De Jong A, Garch FE, Simjee S, Moyaert H, Rose M, Youala M, Siegwart E, VetPath Study Group (2018) Monitoring of antimicrobial susceptibility of udder pathogens recovered from cases of clinical mastitis in dairy cows across Europe: VetPath results. Vet Microbiol 213:73–81Google Scholar
  20. De Vliegher S, Fox LK, Piepers S, McDougall S, Barkema HW (2012) Invited review: mastitis in dairy heifers: nature of the disease, potential impact, prevention, and control. J Dairy Sci 95:1025–1040Google Scholar
  21. Down PM, Bradley AJ, Breen JE, Green MJ (2017) Factors affecting the cost-effectiveness of on-farm culture prior to the treatment of clinical mastitis in dairy cows. Prev Vet Med 145:91–99Google Scholar
  22. Erskine RJ, Wagner S, DeGraves FJ (2003) Mastitis therapy and pharmacology. Vet Clin North Am Food Anim Pract 19(1):109–138 VIGoogle Scholar
  23. Fernandez-Cabezudo MJ, El-Kharrag R, Torab F, Bashir G, George JA, El-Taji H, Al-Ramadi BK (2013) Intravenous administration of manuka honey inhibits tumor growth and improves host survival when used in combination with chemotherapy in a melanoma mouse model. PLoS One 8(2):e55993Google Scholar
  24. Fiordalisi SAL, Honorato LA, Loiko MR, Avancini CAM, Veleirinho MBR, Machado Filho LCP, Kuhnen S (2017) The effects of Brazilian propolis on etiological agents of mastitis and the viability of bovine mammary gland explants. J Dairy Sci 99:2308–2318Google Scholar
  25. Fitzgerald JR (2012) Livestock-associated Staphylococcus aureus: origin, evolution and public health threat. Trends Microbiol 20(4):192–198Google Scholar
  26. Gomes F, Henriques M (2015) Control of bovine mastitis: old and recent therapeutic approaches. Curr Microbiol 72(4):377–382Google Scholar
  27. Han SM, Lee KG, Yeo JH, Hwang SJ, Chenoweth PJ, Pak SC (2009) Somatic cell count in milk of bee venom treated dairy cows with mastitis. J ApiProduct ApiMed Sci 1(4):104–109Google Scholar
  28. Hogeveen H, Huijps K, Lam TJGM (2011) Economic aspects of mastitis: new developments. NZ Vet J 59(1):16–23Google Scholar
  29. Jensen LB, Angulo FJ, Mølbak K, Wegener HC (2008) Chapter: Human health risks associated with antimicrobial use in animals pp. 14-26. In: Guide to antimicrobial use in animals. Guardabassi L, Jensen LB, Kruse H, Blackwell Publishing UKGoogle Scholar
  30. Joshi S, Gokhale S (2006) Status of mastitis as an emerging disease in improved and peri-urban dairy farms in India. Ann NY Acad Sci 1081:74–83Google Scholar
  31. Lafi SQ, Al-Rawashdeh OF, Ereifej KL, Hailat NQ (1994) Incidence of clinical mastitis and prevalence of sub clinical udder infection in Jordan. Prevent Vet Med 18:89–98Google Scholar
  32. Mandal MD, Mandal S (2011) Honey: its medicinal property and antibacterial activity. Asian Pac J Trop Biomed 1(2):154–160Google Scholar
  33. Manyi-Loh C, Mamphweli S, Meyer E, Okoh A (2018) Antibiotic use in agriculture and its consequential resistance in environmental sources: potential public health implications. Molecules 23(4):795Google Scholar
  34. McLoone P, Warnock M, Fyfe L (2016) Honey: an immunomodulatory agent for disorders of the skin. Food Agric Immunol 27(3):338–349Google Scholar
  35. Melchior MB, Vaarkamp H, Fink-Gremmels J (2006) Biofilms: a role in recurrent mastitis infections? Vet J 171(3):398–407Google Scholar
  36. Molan PC, Betts JA (2004) Clinical usage of honey as a wound dressing: an update. J Wound Care 13(8):353–356Google Scholar
  37. NMC (2004) Microbiological procedures for the diagnosis of bovine udder infection and determining milk quality. National Mastitis Council Inc., ArlingtonGoogle Scholar
  38. Olaitan PB, Adeleke EO, Lyabo OO (2007) Honey: a reservoir for microorganisms and an inhibitory agent for microbes. Afr Health Sci 7:159–165Google Scholar
  39. Oliver SP, Murinda SE (2012) Antimicrobial resistance of mastitis pathogens. Vet Clin N Am Food Anim Pract 28(2):165–185Google Scholar
  40. Omara ST (2017) MIC and MBC of honey and gold nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) Coagulase-positive S. aureus isolated from contagious bovine clinical mastitis. J Genet Eng Biotechn 15:219–230Google Scholar
  41. Ott S (2000) Costs of herd-level production losses associated with subclinical mastitis in US dairy cows. In: proceedings of the 38th Annual Meeting of the National Mastitis Council. Madison (WI): National Mastitis Council, 152–6Google Scholar
  42. Packer RA (1977) Bovine mastitis caused by Pseudomonas aeruginosa. J Am Vet Med Assoc 170:1166Google Scholar
  43. Patel A, Chauhan BP (2017) Antimicrobial effect of honey on MRSA isolated from pus samples. Int J Drug Res Technol 6(2):58–63Google Scholar
  44. Peton V, Le Loir Y (2014) Staphylococcus aureus in veterinary medicine. Infect Genet Evol 21:602–615Google Scholar
  45. Power E (2003) Gangrenous mastitis in dairy herds. Vet Rec 153:791–792Google Scholar
  46. Radostits OM, Blood DC, Gay CC (1994a) Veteri nary Medicine. 8th Edition, ELBS. Bailliére Tindall. 1763 ppGoogle Scholar
  47. Radostits OM, Leslie KE, Saunders WB, Leslie KE, Fetrow J (1994b) Herd health: food animal production medicine, Saunders WB, Philadelphia, Pa, USA, 2nd editionGoogle Scholar
  48. Radostits OM, Gay CC, Hinchcliff KW, Constable PD (2007) Veterinary medicine: a textbook of the disease of cattle, horses, sheep, pigs and goats. Elsevier Ltd, LondonGoogle Scholar
  49. Ruegg PL (2003) Investigation of mastitis problems on farms. Vet Clin Food Anim Pract 19(1):47–73Google Scholar
  50. Saidi R, Khelef D, Kaidi R (2013) Subclinical mastitis in cattle in Algeria: frequency of occurrence and bacteriological isolates. J S Afr Vet Assoc 84(1):1–5Google Scholar
  51. Samarghandian S, Farkhondeh T, Samini F (2017) Honey and health: a review of recent clinical research. Pharm Res 9(2):121–127Google Scholar
  52. Santana HF, Barbosa AA, Ferreira SO, Mantovani HC (2012) Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows. World J Microbiol Biotechnol 28(2):485–491Google Scholar
  53. Scali F, Camussone C, Calvinho LF, Cipolla M, Zecconi A (2015) Which are important targets in development of S. aureus mastitis vaccine? Res Vet Sci 100:88–99Google Scholar
  54. Seegers H, Fourichon C, Beaudeau F (2003) Production effects related to mastitis and mastitis economics in dairy cattle herds. Vet Res 34:475–491Google Scholar
  55. Sherlock O, Dolan A, Rahma A, Power A, Gethin G, Cowman S, Humphrey H (2010) Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. BMC Complement Altern Med 10(1):47Google Scholar
  56. Smith R, Coast J (2013) The true cost of antimicrobial resistance. BMJ 346:f1493.  https://doi.org/10.1136/bmj.f1493
  57. Subrahmanyam M (1991) Topical application of honey treatment of burns. Br J Surg 78(4):497–498Google Scholar
  58. Sutra L, Poutrel B (1994) Virulence factors involved in the pathogenesis of bovine intramammary infections due to Staphylococcus aureus. J Med Microbiol 40(2):79–89Google Scholar
  59. Tacconelli E, Magrini N (2017) Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics 2017. World Health Organization, Geneva, Switzerland. http://www.who.int/medicines/publications/WHO-PPL-Short_Summary_25Feb-ET_NM_WHO.pdf
  60. Wahba NM, El Nisr NA, Sayed SM, Abd Ellah MR, Abd El-Hafeez MM, Aamer AA (2011) Intramammary honey infusion: a new trend in the management of bovine subclinical mastitis. J Anim Vet Adv 10(20):2740–2744Google Scholar

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© Springer Nature B.V. 2019

Authors and Affiliations

  • Mokhtar Benhanifia
    • 1
    Email author
  • Abdelhanine Ayad
    • 2
  • Wessam Mohamed
    • 3
  1. 1.Department of Agricultural Science, Faculty of Natural and Life SciencesMascara UniversityMascaraAlgeria
  2. 2.Department of Biological Sciences of Environment, Faculty of Natural and Life SciencesUniversity A. MiraBejaiaAlgeria
  3. 3.Green Chemistry DepartmentNational Research CenterCairoEgypt

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