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Effects of Agricultural Pollutants on Stress Hormones and Viral Infection in Larval Salamanders

  • Drew R. DavisEmail author
  • Katie J. Ferguson
  • Matthew S. Schwarz
  • Jacob L. Kerby
General Wetland Science

Abstract

Declines in amphibians are a global problem, influenced by complex local factors. While many factors contribute to these declines, much attention has been focused on the roles of contaminants and pathogens. Throughout eastern South Dakota, row-crop farming has contributed to habitat degradation for many amphibians, often through increases in environmental contaminants. For two years we visited four wetlands (two reference wetlands, two agricultural wetlands) to measure water-borne corticosterone (CORT) release rates and ranavirus in larval Western Tiger Salamander (Ambystoma mavortium). We found that both water-borne CORT release rates and ranavirus infection load were greater in larval salamanders from agricultural wetlands compared to reference wetlands. We also found that water-borne CORT release rates were greater in ranavirus-infected individuals compared to uninfected individuals and that water-borne CORT is positively correlated with ranavirus infection load. Though the causal relationships among contaminants, CORT, and ranavirus infection are difficult to determine, chronically elevated CORT is known to be immunosuppressive and may result in high infection loads. This study further describes the negative effects of crop production on amphibian health, provides the first evidence of ranavirus in South Dakota, and supports the use of water-borne CORT as a biomarker of amphibian population health in row-crop landscapes.

Keywords

Agriculture Amphibian conservation Contaminants Corticosterone Habitat degradation Ranavirus 

Notes

Acknowledgements

We thank A. Millikin and A. Koch for field assistance, United States Fish and Wildlife Service (USFWS) and N. Hansen for permission to conduct research on Waterfowl Production Areas in the Madison Wetland Management District, C. Gabor for logistical insight, and both C. Heimerl and E. Dowd Stukel for continued support of our herpetological research program in the state. Fieldwork was conducted under an approved South Dakota Game, Fish and Parks (SDGFP) Scientific Collector’s Permit (2013_#3) issued to DRD and under an approved University of South Dakota (USD) IACUC protocol (#22-04-13-16C). Funding was provided, in part, by the USFWS Environmental Contaminants On-Refuge Program, a SDGFP State Wildlife Grant, an American Society of Ichthyologists and Herpetologists Gaige Award, a USD Research and Creative Activity Grant, and through support from the USD U. Discover Summer Scholarship. Helpful comments on this manuscript were provided by M. Dixon, J. Farkas, M. Jarchow, D. Swanson, J. Voyles, and two anonymous reviewers.

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Copyright information

© Society of Wetland Scientists 2019

Authors and Affiliations

  1. 1.Department of BiologyUniversity of South DakotaVermillionUSA
  2. 2.School of Earth, Environmental, and Marine SciencesUniversity of Texas Rio Grande ValleySouth Padre IslandUSA
  3. 3.South Dakota Field OfficeU.S. Fish and Wildlife ServicePierreUSA

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