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Acta Oceanologica Sinica

, Volume 38, Issue 6, pp 46–53 | Cite as

mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene

  • Wanjuan Li
  • Huan Zhao
  • Fuyang Ba
  • Shaojuan Li
  • Xiupeng Sun
  • Dazuo YangEmail author
  • Yibing ZhouEmail author
Article
  • 6 Downloads

Abstract

Rapid amplification of cDNA ends (RACE) and real-time polymerase chain reaction (RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene (BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed U-shaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.

Key words

Marphysa sanguinea CYP4 benzo[a]pyrene toxicity effect 

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References

  1. Alam M A, Gomes A, Sarkar S K, et al. 2010. Trace metal bioaccumulation by soft-bottom polychaetes (Annelida) of Sundarban mangrove wetland, India and their potential use as contamination indicator. Bulletin of Environmental Contamination and Toxicology, 85(5): 492–496Google Scholar
  2. Bach L, Palmqvist A, Rasmussen L J, et al. 2005. Differences in PAH tolerance between Capitella species: underlying biochemical mechanisms. Aquatic Toxicology, 74(4): 307–319Google Scholar
  3. Baldwin W S, Marko P B, Nelson D R. 2009. The cytochrome P450 (CYP) gene superfamily in Daphnia pulex. BMC Genomics. 10. 169Google Scholar
  4. Barakat A O, Mostafa A, Wade T L, et al. 2011. Distribution and characteristics of PAHs in sediments from the Mediterranean coastal environment of Egypt. Marine Pollution Bulletin, 62(9): 1969–1978Google Scholar
  5. Chen Wanping, Lee M K, Jefcoate C, et al. 2014. Fungal cytochrome p450 monooxygenases: their distribution, structure, functions, family expansion, and evolutionary origin. Genome Biology and Evolution, 6(7): 1620–1634Google Scholar
  6. Chen Xue, Zhou Yibing, Yang Dazuo, et al. 2012. CYP4 mRNA expression in marine polychaete Perinereis aibuhitensis in response to petroleum hydrocarbon and deltamethrin. Marine Pollution Bulletin, 64(9): 1782–1788Google Scholar
  7. Dean H K. 2008. The use of polychaetes (Annelida) as indicator species of marine pollution: a review. Revista de Biologia Tropical, 56(S4): 11–38Google Scholar
  8. Feng Chenglian, Lei Bingli, Wang Zijian. 2009. Preliminary ecological risk assessment of polycyclic aromatic hydrocarbons in main rivers of China. China Environmental Science (in Chinese), 29(6): 583–588Google Scholar
  9. Fisher T, Crane M, Callaghan A. 2003. Induction of cytochrome P-450 activity in individual Chironomus riparius Meigen larvae exposed to xenobiotics. Ecotoxicology and Environmental Safety, 54(1): 1–6Google Scholar
  10. Guo Hui, Xian Jian'an, Li Bin, et al. 2013. Gene expression of apoptos-is-related genes, stress protein and antioxidant enzymes in hemocytes of white shrimp Litopenaeus vannamei under nitrite stress. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 157(4): 366–371Google Scholar
  11. Han J, Won E J, Hwang D S, et al. 2014. Crude oil exposure results in oxidative stress-mediated dysfunctional development and reproduction in the copepod Tigriopus japonicus and modulates expression of cytochrome P450 (CYP) genes. Aquatic Toxicology. 152. 308–317Google Scholar
  12. Hong S, Khim J S, Ryu J, et al. 2012. Two years after the Hebei Spirit oil spill: residual crude-derived hydrocarbons and potential AhR-mediated activities in coastal sediments. Environmental Science & Technology, 46(3): 1406–1414Google Scholar
  13. Jernelöv A. 2010. The threats from oil spills: Now, then, and in the future. Ambio, 39(5-6): 353–366Google Scholar
  14. Jørgensen A, Giessing A M B, Rasmussen L J, et al. 2008. Biotransformation of polycyclic aromatic hydrocarbons in marine poly-chaetes. Marine Environmental Research, 65(2): 171–186Google Scholar
  15. Jørgensen A, Rasmussen L J, Andersen O. 2005. Characterisation of two novel CYP4 genes from the marine polychaete Nereis virens and their involvement in pyrene hydroxylase activity. Biochemical and Biophysical Research Communications, 336(3): 890–897Google Scholar
  16. Kirischian N L, Wilson J Y. 2012. Phylogenetic and functional analyses of the cytochrome P450 famil. 4. Molecular Phylogenetics and Evolution, 62(1): 458–471Google Scholar
  17. Kraugerud M, Doughty R W, Lyche J L, et al. 2012. Natural mixtures of persistent organic pollutants (POPs) suppress ovarian follicle development, liver vitellogenin immunostaining and hepatocyte proliferation in female zebrafish (Danio rerio). Aquatic Toxicology, 116-117: 16–23Google Scholar
  18. Lewis C, Watson G J. 2012. Expanding the ecotoxicological toolbox: the inclusion of polychaete reproductive endpoints. Marine Environmental Research. 75. 10–22Google Scholar
  19. Liu Fang, Jiang Hongling, Ye Songqing, et al. 2010. The Arabidopsis P450 protein CYP82C2 modulates jasmonate-induced root growth inhibition, defense gene expression and indole glucosinolate biosynthesis. Cell Research, 20(5): 539–552Google Scholar
  20. Lyche J L, Grześ I M, Karlsson C, et al. 2013. Parental exposure to natural mixtures of POPs reduced embryo production and altered gene transcription in zebrafish embryos. Aquatic Toxicology. 126. 424–434Google Scholar
  21. Lyche J L, Nourizadeh-Lillabadi R, Karlsson C, et al. 2011. Natural mixtures of POPs affected body weight gain and induced transcription of genes involved in weight regulation and insulin signaling. Aquatic Toxicology, 102(3-4): 197–204Google Scholar
  22. Martins M, Costa P M, Ferreira A M, et al. 2013. Comparative DNA damage and oxidative effects of carcinogenic and non-carcinogenic sediment-bound PAHs in the gills of a bivalve. Aquatic Toxicology, 142-143: 85–95Google Scholar
  23. Miao Jingjing, Pan Luqing, Liu Na, et al. 2011. Molecular cloning of CYP4 and GSTpi homologues in the scallop Chlamys farreri and its expression in response to benzo[a]pyrene exposure. Marine Genomics, 4(2): 99–108Google Scholar
  24. Musale A S, Desai D V. 2011. Distribution and abundance of macrobenthic polychaetes along the South Indian coast. Environmental Monitoring and Assessment, 178(1-4): 423–436Google Scholar
  25. Nelson D R. 2011. Progress in tracing the evolutionary paths of cytochrome P450. Biochimica et Biophysica Acta (BBA)—Proteins and Proteomics, 1814(1): 14–18Google Scholar
  26. Ohtsuki S, Schaefer O, Kawakami H, et al. 2012. Simultaneous absolute protein quantification of transporters, cytochromes P450, and UDP-glucuronosyltransferases as a novel approach for the characterization of individual human liver: comparison with mRNA levels and activities. Drug Metabolism and Disposition, 40(1): 83–92Google Scholar
  27. Onozato M, Sugawara T, Nishigaki A, et al. 2012. Study on the Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) in the Excrement of Marphysa sanguinea. Polycyclic Aromatic Compounds, 32(2): 238–247Google Scholar
  28. Pan Luqing, Liu Na, Xu Chaoqun, et al. 2011. Identification of a novel P450 gene belonging to the CYP4 family in the clam Ruditapes philippinarum, and analysis of basal- and benzo(a)pyrene-induced mRNA expression levels in selected tissues. Environmental Toxicology and Pharmacology, 32(3): 390–398Google Scholar
  29. Rewitz K F, Kjellerup C, Jørgensen A, et al. 2004. Identification of two Nereis virens (Annelida: Polychaeta) cytochromes P450 and induction by xenobiotics. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 138(1): 89–96Google Scholar
  30. Scornaienchi M L, Thornton C, Willett K L, et al. 2010. Functional differences in the cytochrome P450 1 family enzymes from Zebrafish (Danio rerio) using heterologously expressed proteins. Archives of Biochemistry and Biophysics, 502(1): 17–22Google Scholar
  31. Sim M S, Jo I J, Song H G. 2010. Acute health problems related to the operation mounted to clean the Hebei Spirit oil spill in Taean, Korea. Marine Pollution Bulletin, 60(1): 51–57Google Scholar
  32. Song Yingying, Yuan Xiutang, Zhang Shengli, et al. 2011. Single and Joint Toxic Effects of Benzo(a)pyrene and Cadmium on Development of three-setiger Juvenile of Polychaete Pernereis aibuhitensis Grube. Marine Environmental Science (in Chinese), 30(3): 333–336Google Scholar
  33. Tobiszewski M, Namieśnik J. 2012. PAH diagnostic ratios for the identification of pollution emission sources. Environmental Pollution. 162. 110–119Google Scholar
  34. Tsai W T, Mi H H, Chang Yuanming, et al. 2007. Polycyclic aromatic hydrocarbons (PAHs) in biocrudes from induction-heating pyrolysis of biomass wastes. Bioresource Technology, 98(5): 1133–1137Google Scholar
  35. Uno S, Sakurai K, Nebert D W, et al. 2014. Protective role of cytochrome P450 1A1 (CYP1A1) against benzo[a]pyrene-induced toxicity in mouse aorta. Toxicology. 316. 34–42Google Scholar
  36. Won E J, Rhee J S, Shin K H, et al. 2013. Complete mitochondrial genome of the marine polychaete, Perinereis nuntia (Polychaeta, Nereididae). Mitochondrial DNA, 24(4): 342–343Google Scholar
  37. Xu Jun, Wang Xinyu, Guo Wangzhen. 2015. The cytochrome P450 superfamily: Key players in plant development and defense. Journal of Integrative Agriculture, 14(9): 1673–1686Google Scholar
  38. Zanette J, Goldstone J V, Bainy A C D, et al. 2010. Identification of CYP genes in Mytilus (mussel) and Crassostrea (oyster) species: first approach to the full complement of cytochrome P450 genes in bivalves. Marine Environmental Research, 69(Suppl 1): S1–S3Google Scholar
  39. Zhao Huan, Wang Yixiao, Yang Dazuo, et al. 2016. An analysis of genetic diversity in Marphysa sanguinea from different geographic populations using ISSR polymorphisms. Biochemical Sys-tematics and Ecology. 64. 65–69Google Scholar
  40. Zheng Shenli, Chen Bin, Qiu Xiaoyan, et al. 2013. Three novel cytochrome P450 genes identified in the marine polychaete Peri-nereis nuntia and their transcriptional response to xenobiotics. Aquatic Toxicology, 134-135: 11–22Google Scholar
  41. Zhou Chi, Li Chunhou, Zhang Weimin, et al. 2010a. CYP4 gene cloning and expression level analysis of Perna viridis. Journal of Tropical Oceanography (in Chinese), 29(4): 82–88Google Scholar
  42. Zhou Xiaojie, Sheng Changfa, Li Mei, et al. 2010b. Expression responses of nine cytochrome P450 genes to xenobiotics in the cotton bollworm Helicoverpa armigera. Pesticide Biochemistry and Physiology, 97(3): 209–213Google Scholar

Copyright information

© Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning ProvinceDalian Ocean UniversityDalianChina
  2. 2.Marine and Fisheries Technology Center of Pan Shan CountryPanjinChina

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