Acta Oceanologica Sinica

, Volume 37, Issue 12, pp 78–84 | Cite as

Next-generation sequencing revealed specific microbial symbionts in Porites lutea with pigment abnormalities in North Sulawesi, Indonesia

  • Danyun Ou
  • Bin ChenEmail author
  • Tri Aryono Hadi
  • Suharsono
  • Wentao Niu
  • Yustian Rovi Alfiansah


Bacterial diseases affecting corals pose an enormous threat to the health of coral reefs. The relationship between certain bacterial species and coral diseases remain largely unknown. Pigment abnormalities are common in Porites lutea. Here we used Illumina 16S rRNA gene sequencing to analyze the bacterial communities associated with healthy P. lutea and P. lutea with pigment abnormalities. We observed an increase of alpha diversity of the bacterial community of P. lutea with pigment abnormalities, relative to healthy corals. We then identified changes in the abundance of individual operational taxonomic units (OTUs) between pigmented and healthy corals. We were able to identify eight OTUs associated with pigment abnormalities, which are possibly the causative agents of pigment abnormalities.

Key words

Porites lutea pigment abnormalities next-generation sequencing 16S rRNA bacterial diversity 


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The authors thank Bai Renao for contributing to DNA extraction, and Novogene Ltd. for helping with Illumina DNA sequencing.


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Copyright information

© The Chinese Society of Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Danyun Ou
    • 1
  • Bin Chen
    • 1
    Email author
  • Tri Aryono Hadi
    • 2
  • Suharsono
    • 2
  • Wentao Niu
    • 1
  • Yustian Rovi Alfiansah
    • 2
  1. 1.Third Institute of OceanographyMinistry of Natural ResourcesXiamenChina
  2. 2.Research Center for OceanographyIndonesian Institute of SciencesJakartaIndonesia

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