Journal of Physiology and Biochemistry

, Volume 75, Issue 3, pp 275–283 | Cite as

Effects of resveratrol and its analogue pterostilbene, on NOV/CCN3 adipokine in adipose tissue from rats fed a high-fat high-sucrose diet

  • J. Trepiana
  • S. Gómez-Zorita
  • Alfredo Fernández-QuintelaEmail author
  • M. González
  • M. P. Portillo
Original Article


Nephroblastoma overexpressed protein, also called NOV/CCN3, is an adipokine which is present in various tissues and recently linked to obesity. The objective of the study was to determine the effect of resveratrol and pterostilbene on NOV/CCN3 in adipose tissue from rats fed an obesogenic diet. Thirty-six male Wistar rats were split into four groups (n = 9): fed a standard diet (CC), high-fat high-sucrose (HFS) diet supplemented with resveratrol (RSV; 30 mg/kg/day) or with pterostilbene (PT; 30 mg/kg/day), or without phenolic supplementation (HFS). Rats were sacrificed after 6 weeks of treatment, and adipose tissue (white and brown) from different anatomical locations were dissected. Then, Nov/ccn3 gene and protein expression and the adipogenic genes, Ucp-1 and Pgc-1a, expressions were studied. Increased weight of white adipose tissues was found in rats fed the HFS diet. Whereas resveratrol-treated rats showed reduced internal and total adipose tissue weights, pterostilbene-treated rats showed reduced subcutaneous, internal and total adipose depots. Nov/ccn3 gene expression decreased in epididymal and interscapular brown depot in rats fed HFS diet when compared with the control group. Regarding the phenolic compounds, resveratrol prompted a Nov/ccn3 gene expression increase in epididymal fat tissue, whereas pterostilbene reduced its protein expression compared with the obese group. However, these phenolic compounds did not affect NOV/CCN3 expression in brown depot. NOV/CCN3 seems to be involved in weight changes in epididymal adipose tissue under obesogenic feeding, but not in subcutaneous, acting as a protective mechanism counteracting the fattening effect of the diet. To our knowledge, this is the first study analyzing whether NOV/CCN3 is involved in the anti-obesity effect of resveratrol and pterostilbene. Our results suggest that this is not the case.


NOV/CCN3 Resveratrol Pterostilbene Phenolic compounds Adipose tissue Obesity 


Funding information

This study was supported by grants from the Instituto de Salud Carlos III (CIBERObn), Government of the Basque Country (IT-572-13).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© University of Navarra 2019

Authors and Affiliations

  1. 1.Nutrition and Obesity Group, Department of Nutrition and Food ScienceUniversity of the Basque Country (UPV/EHU) and Lucio Lascaray Research InstituteVitoriaSpain
  2. 2.Biomedical Research Networking Centres, Physiopathology of Obesity and Nutrition (CIBERobn)Institute of Health Carlos IIIMadridSpain
  3. 3.Department Nutrición y Bromatología, Facultad de FarmaciaPaseo de la Universidad 7VitoriaSpain
  4. 4.Nutrition and Food Science Department, Faculty of Biochemistry and Biological SciencesNational University of Litoral and National Scientific and Technical Research Council (CONICET)Santa FeArgentina

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