Journal of Physiology and Biochemistry

, Volume 74, Issue 3, pp 431–439 | Cite as

Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat model

  • Dong Wu
  • Yu-gang Cheng
  • Xin Huang
  • Ming-wei Zhong
  • Shao-zhuang Liu
  • San-yuan Hu
Original Article


Ameliorated renal function has been reported after bariatric surgery, but the mechanisms underlying this phenomenon are not well-studied. To investigate whether the long non-coding RNA (lncRNA) MALAT1 mediates the amelioration of diabetic nephropathy after duodenal-jejunal bypass (DJB) surgery, rats were assigned randomly into four groups: diabetic (DM) group, DM with DJB surgery group, DM with sham surgery group, and healthy control group. Food intake, body weight, oral glucose tolerance test (OGTT), urine albumin excretion rate (UAER), and glomerular filtration rate (GFR) were measured and histological examination of renal sections was performed. For in vitro study, HK-2 cells were cultured under various glucose concentrations following MALAT1 siRNA transfection. Expression levels of MALAT1, SAA3, IL-6, and TNF-α in rat renal tissues or HK-2 cell lines were evaluated by qRT-PCR and/or ELISA. Results showed DJB surgery improved the renal function of diabetic rats, as indicated by ameliorated UAER and GFR and attenuated glomerular hypertrophy. Expression of MALAT1 and its downstream target SAA3 was significantly downregulated in renal tissues after DJB, which in turn decreased the expression of the pro-inflammatory cytokines IL-6 and TNF-α. Knockdown of MALAT1 in HK-2 cell lines further confirmed that expression levels of SAA3, IL-6, and TNF-α were regulated by MALAT1 under both low- and high-glucose conditions. Our findings suggest that MALAT1 is implicated in the improvement of renal function after DJB through regulation of its downstream targets SAA3, IL-6, and TNF-α.


Diabetes mellitus Duodenal-jejunal bypass Long non-coding RNA MALAT1 Renal function 



This study was supported by grants from the National Natural Science Foundation of China (grant No. 81600059, 81471019/H0712), the Natural Science Foundation of Shandong Province (grant No. ZR2014HQ004), and the Fundamental Research Funds of Shandong University (grant No. 2014QLKY22).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© University of Navarra 2018

Authors and Affiliations

  • Dong Wu
    • 1
  • Yu-gang Cheng
    • 1
  • Xin Huang
    • 1
  • Ming-wei Zhong
    • 1
  • Shao-zhuang Liu
    • 1
  • San-yuan Hu
    • 1
  1. 1.Department of General SurgeryQilu Hospital of Shandong UniversityJinanPeople’s Republic of China

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