Impact of Zinc Stress on Biochemical and Biophysical Parameters in Coffea Arabica Seedlings
Zinc is an essential micronutrient for the healthy development of plants, since its insufficient and supraoptimal doses can disrupt the metabolism and biomass production. We aimed to investigate the physiological responses of coffee seedlings to Zn deficiency and excess. Six-month-old seedlings were transferred to plastic pots containing a nutrient solution. The treatments were control (0.03 ppm), zinc deficiency (0.00 ppm), and zinc excess (0.12 ppm). The evaluations were performed in leaves and roots at the beginning of the treatments and after 30 and 60 d of treatments. Zn deficiency and excess increased the production of hydrogen peroxide, antioxidant enzymes activity, ascorbate, and lipid peroxidation contents. The imbalance in zinc nutrition reduced total chlorophyll content and increased carotenoids content throughout the experimental period. Lower biomass and proline accumulation were observed only for deficient seedlings at the end of the experiment. The characteristics analyzed showed that zinc deficiency caused greater damage to the Coffea arabica plants of (Catuaí cultivar) than zinc excess.
Key wordsPlant nutrition coffee antioxidant metabolism proline plant growth
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We especially thank the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for funding and Dr. João Paulo R. Alves Delfino Barbosa for assisting us in this project.
- Arakawa N, Tsutsumi K, Sanceda NG, Kurata T, Inagaki C. 1981. A Rapid and Sensitive Method for the Determination of Ascorbic Acid using 4,7-Diphenyl-l,10-phenanthroline. Agric. Biol. Chem. 45(5): 1289–1290Google Scholar
- Bandurska H, Niedziela J, Pietrowska-Borek M, Nuc K, Chadzinikolau T, Radzikowska D 2017. Regulation of proline biosynthesis and resistance to drought stress in two barley (Hordeum vulgare L.) genotypes of different origin. Plant Physiol. 118: 427–437Google Scholar
- Clemente JM. 2014. Boron, copper and zinc effects on photosynthesis, enzymatic activity, nutritional status, production, chemical composition and cup quality of coffee. PhD diss., Universidade Federal de Viçosa, ViçosaGoogle Scholar
- Epstein E, Bloom AP. 2006. Princípios e perspectivas. Ed2.: Planta LondrinaGoogle Scholar
- Faquin V. 2002. Diagnose do estado nutricional das plantas. Lavras: UFLA/FAEPE, 1, 77Google Scholar
- Feigl G, Lehotai N, Molnár Á, Ördög A, Rodríguez-Ruiz M, Palma JM, et al. 2014. Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of two Brassica species with different sensitivity to zinc stress. Ann. Bot. 116(4): 613–25CrossRefGoogle Scholar
- Ferreira DF. 2011. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia. FapUNIFESP (Scielo), Dec;35(6): 1039–42Google Scholar
- Guimarães RJ, Mendes ANG, Souza CAS. 2002. Cafeicultura. Lavras: UFLA/FAEPEGoogle Scholar
- Hoagland DR, Arnon DI. 1950. The water culture method for growing plants without soil. Calif. Agric. Exp. Stat.. 347:1–32Google Scholar
- Karuppanapandian TJC, Moon C, Kim K, Kim W. 2011. Reactive oxygen species in plants: their generation, signal transduction, and scavenging mechanisms. Aust. J. Crop Sci. 5(6): 709Google Scholar
- Lichtenthaler HK, Buschmann C. 2001. chlorophylls and carotenoids: measurement and characterization by uv-vis spectroscopy. CPFAC 1(1): F4.3.1–F4.3.8Google Scholar
- Malavolta E. 1997. Avaliação do estado nutricional das plantas: princípios e aplicações. 2. ed. Piracicaba: PotafosGoogle Scholar
- Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell PhysiolGoogle Scholar
- Nazarbeygi E, Yazdi HL, Naseri R, Soleimani R. 2011. The effects of different levels of salinity on proline and A-, B-chlorophylls in Canola. Amer.-Eur. J. Agric. Environ. Sci. 10: 70–74Google Scholar
- Vassilev AA, Nikolova L, Koleva, Lidon F. 2011. effects of excess zn on growth and photosynthetic performance of young bean plants. J. Phytol. 3: 58–62Google Scholar
- Zabini AV, Martinez, HEP, Finger FL, Silva CA. 2007. Con-centração de micronutrientes e características bioquímicas de progênies de cafeeiros (Coffea arabica L.) eficientes no uso de zinco. Biosci. J. 23(4): 95–103Google Scholar