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Journal of Crop Science and Biotechnology

, Volume 22, Issue 1, pp 47–54 | Cite as

Response of Selected Kenyan Rice Cultivars to Infection by Root Knot Nematode (Meloidogyne incognita)

  • John Namu
  • Amos Alakonya
  • Hannah KaruriEmail author
  • Justin Nyaga
  • Joel Masanga
  • Editah Njeri
Research Article
  • 10 Downloads

Abstract

Meloidogyne incognita causes huge yield losses in rice which is the third most important cereal crop in Kenya. The aim of this study was to identify M. incognita-resistant rice cultivars from Kenya and relate the responses to known resistance pathways with OsPR1a, OsPAL1, and OsJAMYB as marker genes in rice. Five rice cultivars BW 196, Basmati 217 (Pishori), Sindano, IR 2793-80-1 (grown in lowland irrigated fields), and NERICA 4 (grown in upland rainfed fields) were evaluated for resistance to M. incognita under greenhouse conditions in two separate trials. The number of nematode eggs, reproduction factor (RF), and the level of galling were determined. The RF was used to select resistant cultivars. There was a significant difference (P < 0.001) in the number of eggs, galling index, and RF among the cultivars. NERICA 4, BW 196, and Sindano were classified as resistant with an RF <1. There was differential expression of the three marker genes between susceptible and resistant cultivars. OsJAMYB gene was up-regulated in leaves of all rice cultivars after 1 and 3 days post inoculation (dpi). OsPAL1 was up-regulated in leaves of all varieties at 3 dpi while OsPR1a was down-regulated in leaves of resistant plants at 1 dpi and 3 dpi. These results provide an insight on sources of M. incognita resistance in Kenyan rice and it also forms an interesting starting point for further studies on defense responses of common rice varieties to root knot nematode infection.

Key words

Defense response Oryza sativa root knot nematode resistance 

Abbreviations

dpi

days post inoculation

GI

Galling index

J2s

Second stage juveniles

KALRO

Kenya Agricultural and Livestock Research Organization

Pf

Final population

Pi

Initial population

RF

Reproduction factor

RKN

Root knot nematodes

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Copyright information

© Korean Society of Crop Science and Springer 2019

Authors and Affiliations

  • John Namu
    • 1
  • Amos Alakonya
    • 1
    • 2
  • Hannah Karuri
    • 3
    Email author
  • Justin Nyaga
    • 3
  • Joel Masanga
    • 4
  • Editah Njeri
    • 5
  1. 1.Institute for Biotechnology ResearchJomo Kenyatta University of Agriculture and TechnologyNairobiKenya
  2. 2.International Institute of tropical AgricultureIbadanNigeria
  3. 3.Department of Biological SciencesUniversity of EmbuEmbuKenya
  4. 4.Plant transformation Laboratory, Department of Biochemistry and BiotechnologyKenyatta UniversityNairobiKenya
  5. 5.Department of BusinessKenyatta UniversityNairobiKenya

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