Development and characterization of 72 SNP markers in Armeniaca sibirica based on transcriptomics
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Armeniaca sibirica L. (Rosaceae) was once an economically important species in China, but the amount of natural germplasm of A. sibirica recently declined dramatically as a consequence of habitat fragmentation and overharvesting; this species has now been listed as endangered in the China Plant Red Data Book. In this study, 72 polymorphic single nucleotide polymorphism (SNP) markers were developed in A. sibirica based on its transcriptome and characterized by genotyping 70 individuals using the SNaPshot method. The minor allele frequency varied from 0.102 to 0.516, and the observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.052 to 0.649, 0.186 to 0.617, and 0.105 to 0.452, respectively. Among these SNPs, seven loci deviated significantly from the Hardy–Weinberg equilibrium after Bonferroni correction (p < 0.05). These novel SNPs can be useful for future population and conservation genetics studies on this species.
KeywordsArmeniaca sibirica Transcriptome sequencing SNP markers Genetic diversity Conservation genetics
This study was funded by the Research Fund for Young Teachers of the College of Forestry of the Inner Mongolia Agricultural University (Grant Number 203-170007), High-Level Talent Introduction Project of the Inner Mongolia Agricultural University (Grant Number NDYB2017-9), Natural Science Foundation of the Inner Mongolia Autonomous Region (Grant Number 2019BS03019), and National Natural Key R&D Program of China (Grant Number 2018YFD1000606-3).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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