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Development and characterization of 72 SNP markers in Armeniaca sibirica based on transcriptomics

  • Wenquan Bao
  • Dun AoEmail author
  • Tana Wuyun
  • Lin Wang
  • Shulan Bai
  • Yu-e Bai
Technical Note
  • 15 Downloads

Abstract

Armeniaca sibirica L. (Rosaceae) was once an economically important species in China, but the amount of natural germplasm of A. sibirica recently declined dramatically as a consequence of habitat fragmentation and overharvesting; this species has now been listed as endangered in the China Plant Red Data Book. In this study, 72 polymorphic single nucleotide polymorphism (SNP) markers were developed in A. sibirica based on its transcriptome and characterized by genotyping 70 individuals using the SNaPshot method. The minor allele frequency varied from 0.102 to 0.516, and the observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.052 to 0.649, 0.186 to 0.617, and 0.105 to 0.452, respectively. Among these SNPs, seven loci deviated significantly from the Hardy–Weinberg equilibrium after Bonferroni correction (p < 0.05). These novel SNPs can be useful for future population and conservation genetics studies on this species.

Keywords

Armeniaca sibirica Transcriptome sequencing SNP markers Genetic diversity Conservation genetics 

Notes

Acknowledgements

This study was funded by the Research Fund for Young Teachers of the College of Forestry of the Inner Mongolia Agricultural University (Grant Number 203-170007), High-Level Talent Introduction Project of the Inner Mongolia Agricultural University (Grant Number NDYB2017-9), Natural Science Foundation of the Inner Mongolia Autonomous Region (Grant Number 2019BS03019), and National Natural Key R&D Program of China (Grant Number 2018YFD1000606-3).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of ForestryInner Mongolia Agricultural UniversityHohhotChina
  2. 2.Key Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and EnvironmentInner Mongolia Agricultural UniversityHohhotChina
  3. 3.Non-Timber Forest Research and Development CenterChinese Academy of ForestryZhengzhouChina

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