Novel hybrid primers for sequencing of the mitochondrial cytochrome b gene in the rabbitfishes (Teleostei: Siganidae)
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We describe novel hybrid primers for the successful amplification and sequencing of the mitochondrial cytochrome b (cytb) gene in the family Siganidae. We aligned 403 cytb sequences from 29 species and found multiple potential conserved hybrid primers (< twofold degeneracy). The best set was selected, and successfully amplified and sequenced ~ 1100 bp of cytb in the Siganidae.
KeywordsAcanthuridae Cytochrome b Hybrid primers mtDNA Siganidae Zanclidae
We would like to thank the following collaborators for facilitating the collection of samples: Dr Philippe Borsa from Institut de Recherche pour le Développement (IRD, France), Dr Nina Wambiji from the Marine & Fisheries Research Institute (Kenya), Shane Abeare from Reef Doctor (Madagascar), Drs Atanasio Brito and R. Mutombene from the Instituto Nacional de lnvestiqacao Pesqueira (Mozambique), Dr Phillip Heemstra and Elaine Heemstra from the South African Institute for Aquatic Biodiversity (South Africa), Adeline Collet from IRD (New Caledonia), Pierre Boblin and Emmanuel Tardy from SPC (New Caledonia), Dr Filippo Giancarlo from Proyek Biokor (Indonesia and West Papua), Dr Emmanuel Tessier from Parc Marin (La Réunion), Dr Mohamed Hassan from SMEL (France), and Emmy Hardman (Schoals Rodrigues). Ilkser Erdem Kiper was supported by a PhD scholarship from the University of Pretoria’s PhD programme; TBH was supported by the University of Pretoria’s research fellowship programme and the University of Pretoria research development programme. This project was partly funded by the Western Indian Ocean Marine Science Association (WIOMSA): MARG I/11/2013 and the South African Department of Science and Technology/National Research Foundation African Coelacanth Ecosystem Programme (ACEP) II. Sampling was funded in part by the project POE 2.10 POCT FED –FEDER ‘Biodiversité de l’Océan Indien’, IRD, SPC and SAIAB.
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Conflict of interest
The authors declare that they have no conflict of interest.