Abstract
Microsatellite markers were developed for the coral reef fish Chaetodon vagabundus using shotgun pyrosequencing. As threats to coral reefs intensify, information on larval connectivity is of increasing value for efficient conservation planning. Here, 11 novel microsatellites were characterized for 192 individuals from Papua New Guinea. The number of alleles per locus ranged from 7 to 32, while observed and expected heterozygosity values varied from 0.214 to 0.903. These markers will be used to study population structure and larval connectivity of this iconic coral reef fish in coral reefs across the Indo-Pacific.
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Acknowledgments
We would like to thank the Bioscience Core Lab of the King Abdullah University of Science and Technology (KAUST) for technical assistance. For general logistic and fieldwork assistance, we thank GP Jones, SR Thorrold, S Planes, HB Harrison, MA Priest, M Srinivasan, and N Tolou. Funding was provided in part by KAUST baseline research funds (to MLB), a KAUST Special Partnership Collaborative Fellowship (to MLB, SR Thorrold, and PS-A), and the Australian Research Council (ARC) (Award FT0990835 to GR Almany) as well as ARC support to GP Jones.
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Saenz-Agudelo, P., Almany, G.R., Mansour, H. et al. Characterization of 11 novel microsatellite markers for the vagabond butterflyfish, Chaetodon vagabundus . Conservation Genet Resour 7, 713–714 (2015). https://doi.org/10.1007/s12686-015-0440-8
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DOI: https://doi.org/10.1007/s12686-015-0440-8