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Characterization of 28 polymorphic SSR loci from Meretrix lamarkii Deshayes

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Abstract

In this study, 28 polymorphic simple sequence repeats (SSR) markers were isolated from an expressed sequence tag (EST) library of Meretrix lamarkii Deshayes. The number of alleles of these 28 EST-SSR markers varied from 2 to 8, with a mean of 3.64 alleles per locus. The observed, expected heterozygosity and polymorphic information content per locus varied from 0.10 to 1.00, 0.10 to 0.86 and 0.09 to 0.81, respectively. No significant linkage disequilibrium between pairs of loci was found, except two of them significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for population genetic structure investigation and marker assisted breeding for M. lamarkii.

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Acknowledgments

This project was financially supported by the marine special scientific fund for non-profit public causes (201205021-5), Major science and technology project of ZheJiang province (2012C12907-4) and National Shellfish Industry Technology System (CARS-48).

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Authors and Affiliations

  1. Zhejiang Mariculture Research Institute, 6-1 Hetong Brodge, Wenzhou, 325000, China

    Bo Liu, Shuang-shuang Teng, Xue-Liang Chai, Yan-Qing Shao, Guo-Qiang Xiao, Jun Fang & Jiong-ming Zhang

  2. Zhejiang Key Laboratory of Exploitation and Reservation of Coastat Bio-resource, Wenzhou, 325000, China

    Bo Liu, Shuang-shuang Teng, Xue-Liang Chai, Yan-Qing Shao, Guo-Qiang Xiao, Jun Fang & Jiong-ming Zhang

  3. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Bo Liu

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  1. Bo Liu
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  2. Shuang-shuang Teng
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  3. Xue-Liang Chai
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  4. Yan-Qing Shao
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  5. Guo-Qiang Xiao
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  6. Jun Fang
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  7. Jiong-ming Zhang
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Corresponding author

Correspondence to Xue-Liang Chai.

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Liu, B., Teng, Ss., Chai, XL. et al. Characterization of 28 polymorphic SSR loci from Meretrix lamarkii Deshayes. Conservation Genet Resour 6, 13–15 (2014). https://doi.org/10.1007/s12686-013-0032-4

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  • DOI: https://doi.org/10.1007/s12686-013-0032-4

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