Callus Extract Mediated Green Synthesis of Silver Nanoparticles, Their Characterization and Cytotoxicity Evaluation Against MDA-MB-231 and PC-3 Cells

  • Satyanarayana BotchaEmail author
  • Subhashini Devi Prattipati


The green synthesis of nanoparticles using callus extract as reducing agent is an eco-friendly, cost-effective approach. In the present study, silver nanoparticles (AgNPs) were prepared using aqueous callus extract of Hyptis suaveolens and their cytotoxic efficacy against cancer cells namely MDA-MB-231 and PC-3 was reported. Callus growth was observed on Murashige and Skoog (MS) medium supplemented with (BAP) benzylaminopurine (0.5 mg/L) + naphthalene acetic acid (NAA) (2 mg/L), and 45-day-old callus was used as a source for synthesis of AgNPs. The synthesis of AgNPs was identified initially by color change (yellow to brown) of the reaction mixture and was further confirmed by characteristic UV–visible surface plasmon resonance peak at 447 nm. The presence of elemental silver in synthesized AgNPs was confirmed through Energy dispersive X-ray spectroscopy (EDS). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis revealed randomly dispersed, spherical shaped AgNPs, with size ranging from 12 to 25 nm, and the mean particle diameter is 18 ± 4 nm. Crystalline and monophasic nature of AgNPs was identified using XRD studies and distinct Bragg diffraction peaks corresponding to the planes of face-centered cubic lattice. The hydrodynamic diameter measured by dynamic light scattering was 207 nm, and it is higher than TEM and XRD analyses. The zeta potential of AgNPs in water was found to be − 24.5 mV, reflecting their higher stability, and FTIR revealed the presence of phenolic compounds and proteins. The cytotoxic effects of AgNPs against MDA-MB-231 and PC-3 cells were evaluated and their IC50 values were found to be 74.66 and 173.21 μg/mL, respectively. The results of the present study clearly demonstrate that the AgNPs prepared from Hyptis suaveolens have the potential to exert cytotoxic effect on cancer cell lines.

Graphical Abstract


Hyptis suaveolens Callus extract Green synthesis Silver nanoparticles Characterization Cytotoxicity 



Silver nanoparticles


Fourier transform infrared spectra


Scanning electron microscopy


Transmission electron microscopy


X-ray diffraction


Dynamic light scattering


Dulbecco’s modified Eagle medium


3-(4,5-Dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide


Funding Information

This work received financial assistance from the University Grants Commission, New Delhi, India, under the scheme of UGC-National Fellowship (F./2015-16/NFO-2015-17-OBC-AND-36898 /(SA-III/Website) for Students of Other Backward Classes (OBC).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Research involving humans and animals statement


Informed consent


Funding statement



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Authors and Affiliations

  1. 1.Department of BiochemistryAndhra UniversityVisakhapatnamIndia

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