The Role of Intracellular Calcium in Changing of ElectricalCharacteristics of Premotor Interneurons in Intact Snails and Snails During Various Forms of Plasticity

  • Dinara I. SilantyevaEmail author
  • Vyatcheslav V. Andrianov
  • Tatiana Kh. Bogodvid
  • Irina B. Deryabina
  • Lyudmila N. Muranova
  • Aliya Kh. Vinarskaya
  • Khalil L. Gainutdinov


It was previously shown that both associative learning and the formation of long-term sensitization led to the increase in excitability of premotor interneurons of the defensive behavior of terrestrial snail Helix lucorum. In the present study, we analyzed the role of intracellular calcium ions in the maintenance of increased excitability in premotor interneurons of terrestrial snail after the formation of a conditioned defensive reflex. It was shown that the increase of the intracellular Ca2+ concentration after adding caffeine to the solution washing the nervous system of the mollusk led to a decrease of the threshold of action potential and to an increase of the critical level of depolarization without a change of the membrane potential of premotor interneurons in both intact and trained snails. The decrease of the intracellular Ca2+ concentration in premotor interneurons by the intracellular injection of (ethylene glycol-bis (2-aminoethylether)-N, N, N, N-tetraacetic acid) (EGTA) resulted in a significant increase of the threshold of generation of the action potential in intact snails. But the values of threshold of generation of the action potential in trained snails after injection of EGTA did not significantly differ from the values of studied parameters before injection. After application of the membrane-penetrating chelator, BAPTA-AM, the changes in the membrane and threshold potentials of premotor interneurons of intact and trained snails were not observed. Our results demonstrated that both the increase and decrease of intracellular Ca2+ concentration were not involved in maintaining the changes of membrane characteristics of premotor interneurons observed after associative learning.


Calcium ions Associative learning Identified neurons Membrane potential Threshold potential Snail 


Funding Information

The work is financially supported by Russian Foundation for Basic Research (grant 18-015-00274).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Research Involving Humans and Animals Statement

All experimental procedures were carried out according with requirements of the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health, Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 and according with guidelines of Kazan Federal University. The terrestrial snails Helix lucorum were used for present experiments. Capture of animals in the wild nature was carried out by competent persons without any pain and distress (Article 9 of Directive 2010/63/EU). Snails were transported asleep and then stored asleep (Article 33 of Directive 2010/63/EU). Prior to the experiments snails were kept for no less than 2 weeks in a glass terrarium in a humid atmosphere at room temperature (Article 33 of Directive 2010/63/EU).

Informed Consent



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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Dinara I. Silantyeva
    • 1
    Email author
  • Vyatcheslav V. Andrianov
    • 1
  • Tatiana Kh. Bogodvid
    • 1
    • 2
  • Irina B. Deryabina
    • 1
  • Lyudmila N. Muranova
    • 1
  • Aliya Kh. Vinarskaya
    • 3
  • Khalil L. Gainutdinov
    • 1
  1. 1.Laboratory of Motor Neurorehabilitation, Scientific and Clinical Center of Precision and Regenerative Medicine, Institute of Fundamental Medicine and BiologyKazan Federal UniversityKazanRussia
  2. 2.Department of Biomedical SciencesVolga Region State Academy of Physical Culture, Sport and TourismKazanRussia
  3. 3.Laboratory of Cellular Neurobiology of Learning, Institute of High Nerve Activity and NeurophysiologyRussian Academy of SciencesMoscowRussia

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