, Volume 9, Issue 4, pp 821–832 | Cite as

Structural Effects of the Syntheticcobalt–Manganese-Zinc Ferrite Nanoparticles (Co0.3Mn0.2Zn0.5Fe2O4 NPs) on DNA and its Antiproliferative Effect on T47Dcells

  • Azadeh HekmatEmail author
  • Ali Akbar Saboury


The purpose of this investigation was to examine the effect of Cobalt-Manganese-Zinc Ferrite nanoparticles (Co0.3Mn0.2Zn0.5Fe2O4 NPs) on DNA structure along with its inhibitory role in the growth of T47D cells. This investigation performed with ultraviolet and fluorescence spectroscopy, zeta potential investigation, circular dichroism (CD) spectroscopy, as well as MTT assay, DAPI staining, and flow cytometry analyses. The ultraviolet and fluorescence results indicated that Co0.3Mn0.2Zn0.5Fe2O4 NPs could form a complex via non-intercalative mechanism. The thermodynamics parameters exhibited that Co0.3Mn0.2Zn0.5Fe2O4 NPs the hydrophobic force plays a role in this interaction. The CD data confirmed that Co0.3Mn0.2Zn0.5Fe2O4 NPs induced the transition of DNA conformation to a compact molecular form so-called Ψ-form, furthermore, DNA was relatively thermally stable in the presence of Co0.3Mn0.2Zn0.5Fe2O4 NPs. The anticancer property of Co0.3Mn0.2Zn0.5Fe2O4 NPs via MTT assay, DAPI staining and flow cytometry analyses verified that this nanoparticle can reduce T47D cells proliferation. Based on this investigation Co0.3Mn0.2Zn0.5Fe2O4 NPs could change the structure of DNA and can affect the cell viability. This study can offer an innovative strategy for designing a new anti-tumor agent.


Cobalt–manganese-zinc ferrite nanoparticles DNA Ψ-form DNA Spectroscopy T47D cells 



We thank Ms. Mina Evini at Institute of Biochemistry and Biophysics of the University of Tehran for technical support.

Compliance with Ethical Standards

Conflict of Interest

The authors declare no conflict of interest.

Research Involving Humans and Animals Statement


Informed Consent


Funding Statement



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Authors and Affiliations

  1. 1.Department of Biology, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Institute of Biochemistry and BiophysicsUniversity of TehranTehranIran

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