Is Astragalus gossypinus Honey a Natural Antibacterial and Cytotoxic Agent? An Investigation on A. gossypinus Honey Biological Activity and Its Green Synthesized Silver Nanoparticles
Nowadays, there are some inconsistent reports on antibacterial and other biological properties of honey. It seems that, depending on the type, geographical location and source of flowers, honey may or may not have antibacterial, anticancer, and wound healing properties. This natural product has a high potential to combat a variety of diseases, especially infectious diseases, however, directly using this substance in therapeutic processes faces some limitations. The aim of this study is to investigate the cytotoxicity and antibacterial behaviors of Astragalus gossypinus honey. Moreover, the potential of this honey, as an interface of natural products and nanobiotechnology, in the green synthesis of silver nanoparticles was investigated and the antibacterial and cytotoxicity properties of nanoparticles were also assessed. Based on the results, the honey does not show significant antibacterial and cytotoxic activity by itself, while it plays an efficient role in the synthesis and functionalization of stable silver nanoparticles (spherical, 42.7 nm). The honey-functionalized nanoparticles show an ambivalent cytotoxic effect against the L-929 cell line. So that they stimulate the viability of the cells at low concentrations, while they show toxicity toward them at concentrations above 50 μg/mL. The nanoparticles also have a good dose-dependent antibacterial activity against E. coli, P. aeruginosa, and S. aureus bacterial strains.
KeywordsAstragalus gossypinus Honey Green synthesis Silver nanoparticles Antibacterial agent Cytotoxic agent
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
Research Involving Humans and Animals Statement
- 1.Dezmirean, G. I., Mărghitaş, L. A., Bobis, O., Dezmirean, D. S., Bonta, V., & Erler, S. (2012). Botanical origin causes changes in nutritional pro fi le and antioxidant activity of fermented products obtained from honey. Journal of Agricultural and Food Chemistry, 60, 8028–8035.CrossRefGoogle Scholar
- 5.Schramm, D. D., Schramm, D. D., Karin, M., Karin, M., Schrader, H. R., Schrader, H. R., Holt, R. R., Holt, R. R., Cardetti, M., Cardetti, M., Keen, C. L., & Keen, C. L. (2003). Honey with high levels of antioxidants can provide protection to healthy human subjects. Journal of Agricultural and Food Chemistry, 12, 1732–1735.CrossRefGoogle Scholar
- 6.Wang, Q., Cai, W. J., Yu, L., Ding, J., & Feng, Y. Q. (2017). Comprehensive profiling of phytohormones in honey by sequential liquid-liquid extraction coupled with liquid chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry, 65, 575–585. https://doi.org/10.1021/acs.jafc.6b04234.CrossRefGoogle Scholar
- 8.Heidari, T., Roozbahani, N., Amiri Farahani, L., Attarha, M., Akbari Torkestani, N., Jamilian, M., & Bekhradi, R. (2013). Does Iranian Astragalus gossypinus honey assist in healing caesarean wounds and scars? European Journal of Internal Medicine, 5, 226–233. https://doi.org/10.1016/j.eujim.2013.01.005.Google Scholar
- 9.Dhand, V., Soumya, L., Bharadwaj, S., Chakra, S., Bhatt, D., & Sreedhar, B. (2016). Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Materials Science and Engineering: C, 58, 36–43. https://doi.org/10.1016/j.msec.2015.08.018.CrossRefGoogle Scholar
- 12.Edison, T. N. J. I., Lee, Y. R., & Sethuraman, M. G. (2016). Green synthesis of silver nanoparticles using Terminalia cuneata and its catalytic action in reduction of direct yellow-12 dye. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 161, 122–129. https://doi.org/10.1016/j.saa.2016.02.044.CrossRefGoogle Scholar
- 13.Khorrami, S., Zarrabi, A., Khaleghi, M., Danaei, M., & Mozafari, M. (2018). Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. International Journal of Nanomedicine, 13, 8013–8024. https://doi.org/10.2147/IJN.S189295.CrossRefGoogle Scholar
- 15.J.E. Sykes, S.C. Rankin (2013). Isolation and identification of aerobic and anaerobic bacteria. Canine and Feline Infectious Diseases, 6, 17-28Google Scholar
- 16.Zarrabi, A., Shokrgozar, M. A., Vossoughi, M., & Farokhi, M. (2014). In vitro biocompatibility evaluations of hyperbranched polyglycerol hybrid nanostructure as a candidate for nanomedicine applications. Journal of Materials Science. Materials in Medicine, 25, 499–506. https://doi.org/10.1007/s10856-013-5094-z.CrossRefGoogle Scholar
- 18.Manikandan, V., Velmurugan, P., Park, J.-H., Chang, W.-S., Park, Y.-J., Jayanthi, P., Cho, M., & Oh, B.-T. (2017). Green synthesis of silver oxide nanoparticles and its antibacterial activity against dental pathogens. 3 Biotech., 7, 72. https://doi.org/10.1007/s13205-017-0670-4.CrossRefGoogle Scholar
- 19.Nayak, D., Ashe, S., Rauta, P. R., Kumari, M., & Nayak, B. (2016). Bark extract mediated green synthesis of silver nanoparticles: Evaluation of antimicrobial activity and antiproliferative response against osteosarcoma. Materials Science and Engineering: C, 58, 44–52. https://doi.org/10.1016/j.msec.2015.08.022.CrossRefGoogle Scholar
- 21.E.K. Baghkheirati, M.B. Bagherieh-najjar, H. Khandan, A. Abdolzadeh, Synthesis and antibacterial activity of stable bio- conjugated nanoparticles mediated by walnut ( Juglans regia ) green husk extract, 8080 (2015). doi: https://doi.org/10.1080/17458080.2015.1090020.
- 23.Vinoj, G., Pati, R., Sonawane, A., & Vaseeharan, B. (2015). In vitro cytotoxic effects of gold nanoparticles coated with functional acyl homoserine lactone lactonase protein from Bacillus licheniformis and their antibiofilm activity against proteus species. Antimicrobial Agents and Chemotherapy, 59, 763–771. https://doi.org/10.1128/AAC.03047-14.CrossRefGoogle Scholar
- 24.Khaleghi, M., Khorrami, S., & Ravan, H. (2019). Identification of Bacillus thuringiensis bacterial strain isolated from the mine soil as a robust agent in the biosynthesis of silver nanoparticles with strong antibacterial and anti-biofilm activities. Biocatalysis and Agricultural Biotechnology, 18, 101047. https://doi.org/10.1016/j.bcab.2019.101047.CrossRefGoogle Scholar
- 25.Z. Abbasi, S. Feizi, E. Taghipour, P. Ghadam, Green synthesis of silver nanoparticles using aqueous extract of dried Juglans regia green husk and examination of its biological properties, (2017) 1–10. doi: https://doi.org/10.1515/gps-2016-0108.
- 28.Mousavi-Khattat, M., Keyhanfar, M., & Razmjou, A. (2018). A comparative study of stability, antioxidant, DNA cleavage and antibacterial activities of green and chemically synthesized silver nanoparticles. Artificial Cells, Nanomedicine, and Biotechnology, 1–10.Google Scholar
- 30.Assadi, Z., Emtiazi, G., & Zarrabi, A. (2018). Novel synergistic activities of tetracycline copper oxide nanoparticles integrated into chitosan micro particles for delivery against multiple drug resistant strains: Generation of reactive oxygen species (ROS) and cell death. Journal of Drug Delivery Science and Technology, 44, 65–70.CrossRefGoogle Scholar
- 31.Radzig, M. A., Nadtochenko, V. A., Koksharova, O. A., Kiwi, J., Lipasova, V. A., & Khmel, I. A. (2013). Antibacterial effects of silver nanoparticles on gram-negative bacteria: influence on the growth and biofilms formation, mechanisms of action. Colloids and Surfaces. B, Biointerfaces, 102, 300–306. https://doi.org/10.1016/j.colsurfb.2012.07.039.CrossRefGoogle Scholar
- 32.Kardum, N., & Glibetic, M. (2018). Polyphenols and their interactions with other dietary compounds: implications for human health. Advances in Food and Nutrition Research. https://doi.org/10.1016/BS.AFNR.2017.12.001.
- 33.Li, R., Tsao, R., Yang, C., Liu, H., & Zhu, J. C. Y. (2006). Polyphenolic profiles and antioxidant activities of heartnut ( Juglans ailanthifolia Var . cordiformis ) and Persian walnut ( Juglans regia L.). Journal of Agricultural and Food Chemistry, 54, 8033–8040. https://doi.org/10.1021/jf0612171.CrossRefGoogle Scholar
- 37.Satapathy, S. R., Siddharth, S., Das, D., Nayak, A., & Kundu, C. N. (2015). Enhancement of cytotoxicity and inhibition of angiogenesis in oral cancer stem cells by a hybrid nanoparticle of bioactive Quinacrine and silver: implication of base excision repair cascade. Molecular Pharmaceutics, 12, 4011–4025. https://doi.org/10.1021/acs.molpharmaceut.5b00461.CrossRefGoogle Scholar