, Volume 9, Issue 3, pp 749–757 | Cite as

The Kinetic Parameters of Adsorption of Enzymes Using Carbon-Based Materials Obtained from Different Food Wastes

  • Özkan Demirbaş
  • Mehmet Harbi Çalımlı
  • Buse Demirkan
  • Mehmet Hakkı Alma
  • Mehmet Salih NasEmail author
  • Anish KhanEmail author
  • Abdullah M. AsiriEmail author
  • Fatih ŞenEmail author


In this study, adsorption of catalase enzyme onto activated carbon obtained from apple shell, kinetic parameters, and activation data of adsorption process was investigated. Temperature (15, 25, 36.5, and 45 °C), solution pH (5.5, 7, and 9), initial catalase enzyme concentration (1.10−1, 2.10−1, and 2.5.10−1 g l−1), and ionic strength (1.10−2, 5.10−2, and 7.5.10−2 M) have taken as kinetic parameters for the adsorption of catalase enzyme on activated carbon. In all runs, common parameters of temperature, pH, initial enzyme concentration, and ionic strength were taken as 298 K, pH 7, 0.2 g l−1, and 5.10−2 M, respectively. The results of experiments revealed that the adsorption of catalase enzyme onto activated carbon increased with increasing temperature (15–45 °C), ionic strength, and initial catalase enzyme concentration. However, the adsorption process of catalase on activated carbon was affected negatively by increased pH. The thermodynamic functions such as enthalpy (ΔH), activation energy (Ea), entropy (ΔS), and Gibbs energy (ΔG) were investigated. ΔG, Ea, ΔH, and ΔS were found to be − 70.61, 4.19, − 1.69 kJ mol−1, and 231 J mol−1 K−1 for catalase enzyme adsorption, respectively. The adsorption of the process was investigated using Eyring and Arrhenius equations, and the findings showed that the adsorption kinetic is coherent with the pseudo-second-order model. Eventually, activated carbon can be used as an effective adsorbent for the adsorption of catalase.


Catalase enzyme Adsorption Activated carbon Kinetic parameters 


Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed Consent


Funding Statement


Supplementary material

12668_2019_635_MOESM1_ESM.docx (219 kb)
ESM 1 (DOCX 218 kb)
12668_2019_635_MOESM2_ESM.docx (20 kb)
ESM 2 (DOCX 19 kb)


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Authors and Affiliations

  1. 1.Deparment of Chemistry, Faculty of ScienceBalikesir UniversityBalikesirTurkey
  2. 2.Sen Research Group, Department of Biochemistry, Faculty of Science and LiteratureUniversity of DumlupinarKutahyaTurkey
  3. 3.Tuzluca Vocational SchoolIgdir UniversityIgdirTurkey
  4. 4.Department of Environmental Engineering, Faculty of EngineeringIgdir UniversityIgdirTurkey
  5. 5.Chemistry Department, Faculty of ScienceKing Abdulaziz UniversityJeddahSaudi Arabia
  6. 6.Center of Excellence for Advanced Materials ResearchKing Abdulaziz UniversityJeddahSaudi Arabia

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