Biosynthesis of Silver Nanoparticles from Endophytic Fungi, and its Cytotoxic Activity
Nanoparticle research is currently an area of intense scientific interest due to a wide variety of potential applications in biomedical, optical, and electronic fields. The biological synthesis of nanoparticles includes the synthesis of nanoparticles using microbes which have the capability to absorb the nanoparticles that exist in various forms. In the biosynthesis of metal nanoparticles by a fungus Botryodiplodia theobromae, the fungus mycelium is exposed to the metal salt solution. The change in color of the medium indicates the synthesis of silver nanoparticles. The synthesized AgNPs were structurally characterized by UV-visible spectroscopy, scanning electron microscope (SEM), and Fourier-transform infrared spectroscopy (FTIR) analysis. The size of the silver nanoparticles was ranging from 62.77 –103 nm in the fungal mat and 66.75–111.23 nm in the cell filtrate respectively with the existence of free OH and NH groups, aromatic CH stretch, C–C stretch, unsaturation in the molecule, and C–Cl stretch. The cytotoxic efficiency of the AgNPs on human MCF7 breast cancer cell lines and A549 human lung cancer cell lines was appraised by cell viability assay. The synthesized AgNPs inhibited the propagation of cells at an IC50 concentration of 100 μg/mL. Thus, this study reveals that the green synthesis is an eco-friendly method for production of AgNPs from endophytic fungi which provided a powerful anti-proliferative action on MCF-7 and A549 cell lines, suggesting them as a novel chemotherapeutic agent against human breast and lung cancers.
KeywordsEndophytic fungi Botryodiplodia theobromae Silver nanoparticle SEM MCF-7 A549 MTT assay Cytotoxicity
The authors would like to thank the CNST, Anna University, Chennai, for providing the instrumentation facility.
The authors express their sincere thanks to the Department of Science and Technology (INSPIRE), India, for providing necessary funds to carry out the research work.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 3.Suryanarayanan, T. S., & Thennarasan, S. (2004). Temporal variation in endophyte assemblages of Plumeria rubra leaves. Fungal Diversity., 15, 197–204.Google Scholar
- 6.Mann, S. (2001). Biomineralization: principles and concepts in bioinorganic materials chemistry. Oxford: Oxford University Press.Google Scholar
- 7.Senapati, S., Mandal, D., & Ahmad, A. (2004). Fungus mediated synthesis of silver nanoparticles: a novel biological approach. Indian Journal of Physics A., 78(1), 101–105.Google Scholar
- 11.Vardhana, J., & Kathiravan, G. (2015). Biosynthesis of silver nanoparticles by endophytic fungi Pestaloptiopsis pauciseta isolated from the leaves of Psidium guajava Linn. International Journal of Pharmaceutical Science Review and Research., 31(1), 29–31.Google Scholar
- 15.World Cancer Report 2014. World Health Organization. 2014. pp. Chapter 5.1. ISBN 92-832-0429-8.Google Scholar
- 16.World Cancer Report 2014. World Health Organization. 2014. pp. Chapter 1.1. ISBN 92-832-0429-8.Google Scholar
- 17.Janakiraman, V., Jenifer, M., & Ramarajan, S. (2018). Anti diabetic and anti cancer effects of silver nanoparticles synthesised from Botryodiplodia theobromae- an endophytic fungi isolated from Euphorbia hirta – a weed. International Journal of Pharmaceutical Science Review and Resesrch., 53(1), 72–76.Google Scholar
- 19.Rose, D. P., & Connolly, J. M. (1990). Effects of fatty acids and inhibitors of eicosanoid synthesis on the growth of a human breast cancer cell line in culture. Cancer Research., 50, 7139–7144.Google Scholar