Identification of Secondary Metabolite Gene Clusters in the Genome of Bacillus pumilus Strains 7P and 3-19
- 10 Downloads
Members of Bacillus pumilus species are industrially important microorganisms able to secrete a wide range of hydrolytic enzymes and secondary metabolites. However, the traditional methods (based on phenotypes) used to identify such products are time-consuming and are not very specific. Currently, the availability of whole genome sequencing and bioinformatics tools has become a powerful approach for identification of gene clusters associated with the synthesis of substances useful for biotechnology. In order to estimate the potential industrial significance and application of B. pumilus, genome sequencing of two strains (7P and 3-19) and searching of secondary metabolite clusters were performed using antiSMASH program. These analyses revealed 11 potential gene clusters for the synthesis of bacilysin, lichenysin, bacteriocin, and other substances.
KeywordsWhole genome sequencing Bacillus pumilus Secondary metabolites Bacilysin Lichenysin
This work was performed in accordance with the Russian Government Program of Competitive Growth of Kazan Federal University.
The reported study was funded by RFBR, according to the research project No. 19-08-00853 (A).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflicts of interest.
Research Involving Humans and Animals Statement
- 2.Fravel, D. R. (2005). Commercialization and implementation of biocontrol. Annual Review of Phytopathology, 43, 337–350. https://doi.org/10.1146/annurev.phyto.43.032904.092924.CrossRefGoogle Scholar
- 4.Hollensteiner, J., Poehlein, A., Daniel, R., Liesegang, H., Vidal, S., & Wemheuer, F. (2017). Draft genome sequence of Bacillus pumilus strain GM3FR, an endophyte isolated from aerial plant tissues of Festuca rubra L. Genome Announcements, 5(13), e00085–e00017. https://doi.org/10.1128/genomeA.00085-17.CrossRefGoogle Scholar
- 5.Sharipova, M. R., Balaban, N. P., & Leshchinskaya, I. N. (1994). Production of extracellular alkaline phosphatase from antibiotic resistant strain of Bacillus intermedius. Microbiology, 63(1), 52–58.Google Scholar
- 6.Balaban, N. P., Sharipova, M. R., Usmanova, A. M., Itskovich, E. L., & Leshchinskaia, I. B. (1993). Alkaline extracellular proteinase from Bacillus intermedius. Isolation, purification, and some properties of the enzyme. Biokhimiia, 58, 1923–1928.Google Scholar
- 9.Bertani, G. (1951). Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. Journal of Bacteriology, 62(3), 293–300.Google Scholar
- 13.Müller, S., Garcia-Gonzalez, E., Mainz, A., Hertlein, G., Heid, N. C., Mösker, E., van den Elst, H., Overkleeft, H. S., Genersch, E., & Süssmuth, R. D. (2014). Paenilamicin: structure and biosynthesis of a hybrid nonribosomal peptide/polyketide antibiotic from the bee pathogen Paenibacillus larvae. Angewandte Chemie (International Ed. in English), 53(40), 10821–10825. https://doi.org/10.1002/anie.201404572.CrossRefGoogle Scholar
- 14.Garcia-Gonzalez, E., Müller, S., Hertlein, G., Heid, N., Süssmuth, R. D., & Genersch, E. (2014). Biological effects of paenilamicin, a secondary metabolite antibiotic produced by the honeybee pathogenic bacterium Paenibacillus larvae. Microbiologyopen., 3(5), 642–656. https://doi.org/10.1002/mbo3.195.CrossRefGoogle Scholar
- 18.Koumoutsi, A., Chen, X. H., Henne, A., Liesegang, H., Hitzeroth, G., Franke, P., Vater, J., & Borriss, R. (2004). Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42. Journal of Bacteriology, 186, 1084–1096. https://doi.org/10.1128/JB.186.4.1084-1096.2004.CrossRefGoogle Scholar
- 20.Deleu, M., Paquot, M., & Nylander, T. (2005). Fengycin interaction with lipid monolayers at the air-aqueous interface-implications for the effect of fengycin on biological membranes. Journal of Colloid and Interface Science, 283(2), 358–365. https://doi.org/10.1016/j.jcis.2004.09.036.CrossRefGoogle Scholar