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BioNanoScience

, Volume 9, Issue 1, pp 53–58 | Cite as

Differential Sensitivity of Various Markers of Platelet Activation with Adenosine Diphosphate

  • Giang Le Minh
  • Alina D. Peshkova
  • Izabella A. Andrianova
  • John W. Weisel
  • Rustem I. LitvinovEmail author
Article
  • 30 Downloads

Abstract

A number of techniques have been available to assess platelet activation, but their relative sensitivity is unknown and their usage is variable and not based on any rational criteria. Here, we compared the ability of several techniques based on morphological and biochemical markers to detect the first signs of ADP-induced platelet activation. Scanning electron microscopy of platelets was performed in parallel with flow cytometry to quantify the surface expression of P-selectin (marked by labeled anti-CD62P antibodies), active αIIbβ3-intergrin (assessed by the binding of labeled fibrinogen), and phosphatidylserine (assessed by the binding of labeled Annexin V). When expressed as a fraction of activated platelets, shape changes were the most sensitive to a low ADP concentration compared to the biochemical markers in the following order of sensitivity: morphological changes>fibrinogen binding capacity>P-selectin expression> phosphatidylserine exposure. These results suggest the greater sensitivity of platelet microscopy and the importance of its combination with flow cytometry used to detect surface expression of the molecular markers of platelet activation.

Keywords

Platelet activation Scanning electron microscopy Flow cytometry 

Notes

Funding Information

The work was supported by the NIH grant UO1HL116330 and HL090774, National Science Foundation grant DMR150566, the Program for Competitive Growth at Kazan Federal University, and the Russian Foundation for Basic Research/Republic of Tatarstan grant 18-415-16004.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Giang Le Minh
    • 1
  • Alina D. Peshkova
    • 1
  • Izabella A. Andrianova
    • 1
  • John W. Weisel
    • 2
  • Rustem I. Litvinov
    • 1
    • 2
    Email author
  1. 1.Institute of Fundamental Medicine and BiologyKazan Federal UniversityKazanRussia
  2. 2.Department of Cell and Developmental BiologyUniversity of PennsylvaniaPhiladelphiaUSA

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