Antioxidant and Immune Effects of Water Soluble Polysaccharides Isolated from Cinnamomum verum Bark
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Cinnamomum verum plant is widely used in traditional medicine to reduce inflammation. In the present study, water-soluble polysaccharides were isolated from Cinnamomum verum bark followed by sequential separation from four solvent extracts (hexane, dichloromethane, chloroform and methanol) and analysed for the presence of sugar, protein and different phytochemicals. Crude and purified ethyl acetate fractions of soluble polysaccharides were analysed in vitro using mouse leukemic macrophage (RAW 264.7) cell line and human peripheral blood mononuclear cells (PBMCs). Water-soluble (crude and ethyl acetate purified fractionation) polysaccharides contained glucose, whereas proteins and phytochemicals were absent. Water-soluble polysaccharide fraction had less antioxidant activity as compared to all four solvent extracts. Also, water-soluble polysaccharides (250 μg/ml) did not affect the cell growth, while all other four extracts (250 μg/ml) inhibited the growth of RAW 264.7 cells. Cell growth inhibition was detected when concentration of crude and ethyl acetate fraction polysaccharides was increased, where IC50 values were 1120 ± 27 and 1780 ± 227 μg/mL, respectively. Interestingly, crude and ethyl acetate polysaccharides showed concentration-dependent increase of the cell growth in PBMCs, indicating immunostimulating effect. Our data suggests that the immunological properties of cinnamon may be associated with the presence of polysaccharides.
KeywordsPolysaccharides Peripheral blood mononuclear cells Scavenging effect Phytochemicals MTT assay
Authors would like to acknowledge Dr. J. Rema, Principal scientist from the Indian Institute of Spice Research (IISR), Indian Council of Agricultural Research (ICAR), Kerala, India, for providing bark of Cinnamomum verum and Dr. Akshey Jain from Nitin Nursing Home and Dr. Vandana Goyal, from Rajendra Hospital, Patiala, for providing us the blood needed for carrying out the experiments.
Dr. Albert A. Rizvanov and Dr. Svetlana F. Khaiboullina were supported by the Russian Government Program of Competitive Growth of Kazan Federal University. Dr. Albert A. Rizvanov was personally supported by state assignment 20.5175.2017/6.7 of the Ministry of Education and Science of Russian Federation.
Compliance with Ethical Standards
The study was approved by the institutional ethical committee (IEC).
Conflict of Interest
The authors declare that they have no conflict of interest.
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