Synergistic Antimicrobial and Cytotoxic Potential of Zinc Oxide Nanoparticles Synthesized Using Cassia auriculata Leaf Extract
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The problem of microbial resistance is growing, and search for novel approaches to tackle the problem on multidrug resistance pathogens is the need of the hour. The present investigation involves green synthesis of zinc oxide nanoparticles (ZnO NPs) using Cassia auriculata leaf extract and evaluates its synergistic antimicrobial and cytotoxic effect. The results of various techniques confirmed the formation of ZnO NPs. UV-visible spectrum of ZnO NPs showed maximum peak at 370 nm. The crystalline nature of the ZnO NPs was confirmed by XRD analysis. The SEM analysis revealed that particles were spherical and irregular in shape, and average size of nanoparticles was 68.64 nm. The antimicrobial activity and synergistic antimicrobial activity were evaluated against pathogenic microorganisms. ZnO NPs showed broad spectrum of antimicrobial activity against tested pathogens and enhanced synergistic antimicrobial activity as compared to standard antibiotic. Cytotoxic effect of ZnO NPs was evaluated by MTT assay against HeLa cancer cell line, and ZnO NPs showed dose-dependent cytotoxic activity. The synthesized ZnO NPs possess significant antimicrobial and cytotoxic activity and hence can be used therapeutically as nanomedicine for diagnosis and drug therapy.
KeywordsCassia auriculata Zinc oxide nanoparticles Characterization Synergistic antimicrobial activity Cytotoxic activity
The authors thank the Department of Biosciences (UGC-CAS) for providing excellent research facilities. Ms. Hemali Padalia is thankful to UGC-CAS, and Ms. Pooja Moteriya is thankful to UGC, New Delhi, India, for providing Junior Research Fellowship.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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