BioNanoScience

, Volume 8, Issue 1, pp 196–206 | Cite as

Synergistic Antimicrobial and Cytotoxic Potential of Zinc Oxide Nanoparticles Synthesized Using Cassia auriculata Leaf Extract

Article
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Abstract

The problem of microbial resistance is growing, and search for novel approaches to tackle the problem on multidrug resistance pathogens is the need of the hour. The present investigation involves green synthesis of zinc oxide nanoparticles (ZnO NPs) using Cassia auriculata leaf extract and evaluates its synergistic antimicrobial and cytotoxic effect. The results of various techniques confirmed the formation of ZnO NPs. UV-visible spectrum of ZnO NPs showed maximum peak at 370 nm. The crystalline nature of the ZnO NPs was confirmed by XRD analysis. The SEM analysis revealed that particles were spherical and irregular in shape, and average size of nanoparticles was 68.64 nm. The antimicrobial activity and synergistic antimicrobial activity were evaluated against pathogenic microorganisms. ZnO NPs showed broad spectrum of antimicrobial activity against tested pathogens and enhanced synergistic antimicrobial activity as compared to standard antibiotic. Cytotoxic effect of ZnO NPs was evaluated by MTT assay against HeLa cancer cell line, and ZnO NPs showed dose-dependent cytotoxic activity. The synthesized ZnO NPs possess significant antimicrobial and cytotoxic activity and hence can be used therapeutically as nanomedicine for diagnosis and drug therapy.

Keywords

Cassia auriculata Zinc oxide nanoparticles Characterization Synergistic antimicrobial activity Cytotoxic activity 

Notes

Acknowledgements

The authors thank the Department of Biosciences (UGC-CAS) for providing excellent research facilities. Ms. Hemali Padalia is thankful to UGC-CAS, and Ms. Pooja Moteriya is thankful to UGC, New Delhi, India, for providing Junior Research Fellowship.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Hemali Padalia
    • 1
  • Pooja Moteriya
    • 1
  • Sumitra Chanda
    • 1
  1. 1.Phytochemical, Pharmacological and Microbiological laboratory, Department of Biosciences (UGC-CAS)Saurashtra UniversityRajkotIndia

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