Concentration of Alkaline Hydrogen Peroxide (AHP) Affects the Recycle of the Liquid Fraction in the Pre-treatment and Enzymatic Hydrolysis of Corn Stover

  • Bárbara Ribeiro Alves Alencar
  • Fernanda Leitão Vaz
  • Adauto Gomes Barbosa Neto
  • Katia Aparecida Aquino
  • Everardo Valadares de Sa Barretto Sampaio
  • Rômulo Simões Cezar Menezes
  • Emmanuel Damilano DutraEmail author
Original Paper


Pre-treatment is one of main economic and technological challenges to render feasible the production of biofuels and chemical compounds from lignocellulosic biomass. Alkaline hydrogen peroxide (AHP) is the most used pre-treatment and recycling of its liquid fraction can help reduce production costs. The effects of four AHP concentrations (1, 3.5, 5 and 7.5% v/v) on the recycling performance of the liquid fraction of pre-treated corn stover was evaluated for five consecutive cycles. Delignification rates increased with increasing AHP concentrations in the first cycle: 15, 26, 43 and 76% with 1, 3.5, 5 and 7.5% v/v H2O2, respectively. In the following cycles, the rates decreased linearly reaching less than 40% in the last two recycles. These delignification rates and hemicellulose solubilization were corroborated by spectroscopic analyses with Fourier transformation showing reductions in lignin and hemicellulose absorbance and increases in crystallinity indices. Considering the low delignification rates in the last two cycles, the pre-treated biomasses obtained until the third cycle were submitted to enzymatic hydrolysis at 1:10 solid–liquid ratio. The delignification rates affected the efficiency of the enzymatic hydrolysis at all AHP concentrations and all recycles. The highest AHP concentration (7.5% v/v) was required to efficiently remove lignin and solubilize hemicellulose, maintaining cellulose conversion into glucose greater than 50% up to three recycles. Therefore, the technology of recycling the liquid solution of AHP pre-treatment is recommended with high initial concentrations (7.5% v/v).

Graphic Abstract


Lignocellulosic biomass Pre-treatment Recycle liquid fraction Enzymatic hydrolysis 



The authors acknowledge the researchers Dr. Irapuan Pinheiro and Dr. Rafael Souza, from Universidade de Pernambuco, for making available the liquid chromatograph; CETENE for XRD and FTIR analysis and CNPq, CAPES, FACEPE and MCTI for financial support PEGASUS (Processo 441305/2017-2) and ONDACBC - Observatório Nacional da Dinâmica da Água e do Carbono no Bioma Caatinga (INCT-MCTI/CNPQ/CAPES/FAPs (Processo 465764/2014-2) to this research.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Bárbara Ribeiro Alves Alencar
    • 1
  • Fernanda Leitão Vaz
    • 2
  • Adauto Gomes Barbosa Neto
    • 3
  • Katia Aparecida Aquino
    • 4
  • Everardo Valadares de Sa Barretto Sampaio
    • 2
  • Rômulo Simões Cezar Menezes
    • 2
  • Emmanuel Damilano Dutra
    • 2
    • 5
    Email author
  1. 1.Interdepartmental Research Group in Metabolic Engineering, Department of GeneticsFederal University of PernambucoRecifeBrazil
  2. 2.Research Group on Biomass Energy, Department of Nuclear EnergyFederal University of PernambucoRecifeBrazil
  3. 3.Program of Postgraduation in Genetics, Department of GeneticsFederal University of PernambucoRecifeBrazil
  4. 4.Laboratory of Polymers and Nanotechnology, Department of Nuclear EnergyFederal University of PernambucoRecifeBrazil
  5. 5.Departamento de Energia NuclearUniversidade Federal de PernambucoRecifeBrazil

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