Fermented Cassava Residue Lignin Prepared by Sequential Acid Steam-Explosion and Hot-Alkaline Treatment and Its Antioxidant Properties

  • Wenya WangEmail author
  • Shizhuo Wang
  • Yuqing Pan
  • Xianhong Ouyang
  • Robert J. Linhardt
Original Paper


Fermented cassava residue (FCR) is a solid waste generated in cassava-based ethanol production that constitutes a major environmental challenge and wastes a natural resource. In the present study, a process involving sequential acid steam-explosion and hot-alkaline treatment was developed to prepare an antioxidant lignin in high yield and purity from FCR. Two-dimensional NMR analysis indicates that FCR lignin belongs to a guaiacyl/syringyl/hydroxyphenyl (G/S/H) class of lignin. 31P NMR analysis, after hydroxyl group phosphorylation, indicates that phenolic hydroxyl in FCR lignin attributable to mostly G-OH. One FCR lignin (No. 3) possessed the antioxidant activity similar to that of butylated hydroxytoluene. This FCR lignin may have potential applications as a food antioxidant or in other industrial processes as an antioxidant.

Graphic Abstract

The present study provided an efficient process of sequent acid steam-explosion and hot-alkaline treatment to improve the added value of fermented cassava residue (FCR).


Fermented cassava residue Lignin Steam-explosion Antioxidant activity 



This work was supported by the National Key Research and Development Program of China (No. 2016YFD0400601), State Key Laboratory of Pulp and Paper Engineering (201760), Natural Science Foundation of China (NSFC 21576153).

Compliance with Ethical Standards

Conflict of interest

The authors have no conflict of interest.

Research Involving Human and Animal Rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

12649_2019_862_MOESM1_ESM.docx (374 kb)
Supplementary file1 (DOCX 373 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Wenya Wang
    • 1
    • 2
    • 3
    Email author
  • Shizhuo Wang
    • 1
  • Yuqing Pan
    • 1
  • Xianhong Ouyang
    • 1
  • Robert J. Linhardt
    • 4
    • 5
    • 6
  1. 1.College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijingChina
  2. 2.State Key Laboratory of Pulp and Paper EngineeringSouth China University of TechnologyGuangzhouChina
  3. 3.Amoy-BUCT Industrial Bio-Technovation InstituteBeijing University of Chemical TechnologyAmoyChina
  4. 4.Department of Chemistry and Chemical BiologyRensselaer Polytechnic InstituteTroyUSA
  5. 5.Chemical and Biological EngineeringRensselaer Polytechnic InstituteTroyUSA
  6. 6.Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA

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