Eco-Green Conversion of Watermelon Peels to Single Cell Oils Using a Unique Oleaginous Fungus: Lichtheimia corymbifera AH13
- 144 Downloads
Watermelon peel waste (WPW) is being used for first time in single cell oils (SCOs) production via a promising oleaginous fungus Lichtheimia corymbifera which was isolated from Egyptian ecosystem. Pretreatments of WPW were carried out by mechanical, physical and chemical methods; the most potent pretreatment was selected according to total reducing sugar and total lipid production. Accordingly, the mechanical ptetreatment of WPW was distinctly the best pretreatment method for SCOs production from L. corymbifera. Taguchi design clarified that the most optimal culture conditions were 35 °C and pH 7.0 for 4 days by which the highest potential of SCOs and lipid content was yielded (2.93 gl−1 and 39.56% respectively). Mechanical treatment revealed that the dominant fatty acid was palmitic and oleic acids with 41.98 and 34.65% respectively with appearance of γ linolenic acid (GLA) at low concentration 1.43%. Finally, this study showed that WPW was used as a natural, effective, economic, ecofriendly and integrated substrate without adding any outsource nutrients to produce sustainable SCOs with low cost production.
KeywordsWatermelon peels waste Single cell oils Taguchi design
The authors express their sincere thanks to Faculty of science (Boyes), Al-Azhar University, Cairo, Egypt for providing the necessary research facilities. The authors would like to acknowledge the facilities available at National Research Centre of Egypt.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 20.Ibrahim, S., El Saied, H., Hasanin, M.: Active paper packaging material based on antimicrobial conjugated nano-polymer/amino acid as edible coating. J. King Saud Univ. Sci. (2018)Google Scholar
- 25.FAOSTAT. Food and agriculture organization of the United Nations. Statistics division. http://faostat3.fao.org/browse/Q/QC/E/2016. Accessed 2 Mar 2016
- 30.Folch, J., Lees, M., Stanley, G.S.: A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497–509 (1957)Google Scholar
- 31.Mamatha, S.: Polyunsaturated fatty acids (Pufas) OF MUCOR sp. with special reference to gamma linolenic acid (GLA). University of Mysore (2009)Google Scholar
- 33.Suleiman, W., et al.: Recruitment of Cunninghamella echinulata as an Egyptian isolate to produce unsaturated fatty acids. Res. J. Pharm. Biol. Chem. Sci. 9(1), 764–774 (2018)Google Scholar
- 36.Khalil, A.M.A., Hashem, A.H., Abdelaziz, A.M.: Occurrence of toxigenic Penicillium polonicum in retail green table olives from the Saudi Arabia market. Biocatal. Agric. Biotechnol. 101314 (2019)Google Scholar
- 60.Silveira, C.M.D., Oliveira, M.D.S., Furlong, E.B.: Conteúdo lipídico e perfil em ácidos graxos de farelos submetidos à fermentação por Aspergillus oryzae em estado Sólido (2010)Google Scholar
- 74.Enshaeieh, M., et al.: Selection and optimization of single cell oil production from Rodotorula 110 using environmental waste as substrate. J. Cell Mol. Res. 4(2), 68–75 (2013)Google Scholar