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Potential of Chestnut Wastes for Cosmetics and Pharmaceutical Applications

  • N. Flórez-Fernández
  • M. D. TorresEmail author
  • S. Gómez
  • S. Couso
  • H. Domínguez
Original Paper
  • 6 Downloads

Abstract

The aqueous extraction of Castanea sativa underutilized parts (leaves, burs, husks) was proposed to obtain different soluble and insoluble fractions. The optimum extraction and concentration conditions were selected based on previously published studies. The extracts from leaves and husks were concentrated by membranes and those from burs were refined by adsorption–desorption to concentrate the active phenolic compounds showing radical scavenging capacity. A preliminary screening was made in order to explore the extracts’ pharmaceutical potential, evaluating the cell viability against representative human tumoral cells and the potential as a cosmetic ingredient was also evaluated for their inhibitory activities against collagenase, elastase and tyrosinase. In addition, chestnut husk ashes were used as an alternative source for potash production suitable to formulate potassium soaps with interesting mechanical properties.

Graphic Abstract

Keywords

Castanea sativa Leaves Burs Husks Ashes Phenolics Bioactive properties Potash soaps 

Abbreviations

A549

Epithelial lung adenocarcinoma cells

ABTS

2,2′-Azinobis (3-ethyl-benzothiazoline-6-sulfonate)

BT474

Breast cancer HER2+ cells

CB

Chestnut burs

CBCE

Chestnut burs concentrated extract

CBE

Chestnut burs extract

CH

Chestnut leaves

CHACE

Chestnut husks ashes concentrated of extract

CHAE

Chestnut husks ashes of extract

CHCE

Chestnut husks concentrated extract

CHE

Chestnut husks extract

CLCE

Chestnut leaves concentrated extract

CLE

Chestnut leaves extract

CsL

Castanea sativa Leaves

DMSO

Dimethyl sulfoxide

ECM

Enzymes of the extracellular matrix

EGCG

Epigallocatechin galate

GAE

Gallic acid equivalent

HCT-116

Colon carcinoma cells

HEL

Eritroleukemia cells

HL60

Acute promyelocytic leukemia cells

ICP-MS

Inductively coupled plasma mass spectrometry

KG1

Acute myeloid leukemia cells

L-DOPA

3,4-Dihydroxy-l-phenylalanine

MCF7

Breast cancer ER+ cells

MDA-MB-231

Breast cancer cells

MMP-1

Human recombinant collagenase

MMPs

Matrix metalloproteinases

MTT

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

NCIH460

Lung cancer cells

PBS

Phosphate buffer saline

PSN1

Pancreatic adenocarcinoma cells

ROS

Reactive oxygen species

SKBr3

Breast cancer HER2+ cells

T98G

Caucasian human glioblastoma cells

Notes

Acknowledgements

The authors are grateful to the Xunta de Galicia (FEADER 2016/23B and CINBIO). Both projects were partially funded by the FEDER Program of the European Union (“Unha maneira de facer Europa”). M.D.T. thanks Spanish Ministry of Economy and Competitiveness for her postdoctoral grant (IJCI-2016-27535). N.F.F. and S.G. thank CINBIO (Centro singular de investigación de Galicia accreditation 2016–2019).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Chemical EngineeringUniversity of Vigo (Campus Ourense)OurenseSpain
  2. 2.Amarelante Sociedade Cooperativa Galega. A CorredoiraManzaneda, OurenseSpain

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