Waste and Biomass Valorization

, Volume 10, Issue 12, pp 3545–3555 | Cite as

Evaluation of the Effect of Different Extraction Techniques on Sour Cherry Pomace Phenolic Content and Antioxidant Activity and Determination of Phenolic Compounds by FTIR and HPLC

  • İlhami Okur
  • Cem BaltacıoğluEmail author
  • Erdal Ağçam
  • Hande Baltacıoğlu
  • Hami Alpas
Original Paper


Sour cherry pomace derived from mashed cherries that maintains the color and beneficial properties of the cherries (Prunus cerasus L.). In this study, microwave-assisted extraction (MAE) (900 W for 30, 60 and 90 s.), high hydrostatic pressure (HHP) (400 and 500 MPa for 1, 5 and 10 min at 20 °C) and ultrasonic-assisted extraction (UAE) (for 5,10 and 15 min with a power of 100%) was used as novel processes. Total phenolic content (TPC), morphological changes, antioxidant activity (AA) and structural changes of cherry pomace were measured by the Folin–Ciocalteu assay, high performance liquid chromatography (HPLC), DPPH-scavenging activity, scanning electron microscopy (SEM) and Fourier transform infrared spectrometry (FTIR). According to the results, all novel technologies (MAE-HHP-UAE) increase PC and AA with respect to conventional solvent extraction method (50 °C and 30 min) (CSE). Among novel technologies, MAE (90 s) had highest TPC (275.31 ± 4.26 GAE/100 g FW) and also had highest antioxidant activity (89.9 ± 0.22%). The lowest TPC and AA values were 108.36 ± 3.99 mg gallic acid equivalent (GAE)/100 g fresh weight (FW) and 71.30 ± 1.21% in CSE, respectively. According to FTIR results, there are no significant structural changes in samples when different extraction techniques were applied. Individual phenolics were quantified by using HPLC for different extraction techniques.

Graphic Abstract


Sour cherry pomace High hydrostatic pressure (HHP) Ultrasonic-assisted extraction (UAE) Microwave-assisted extraction (MAE) Fourier transform infrared spectrometry (FTIR) High performance liquid chromatography (HPLC) 



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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • İlhami Okur
    • 1
    • 2
  • Cem Baltacıoğlu
    • 1
    Email author
  • Erdal Ağçam
    • 3
  • Hande Baltacıoğlu
    • 1
  • Hami Alpas
    • 2
  1. 1.Food Engineering Department, Engineering FacultyNigde Omer Halisdemir UniversityNigdeTurkey
  2. 2.Food Engineering Department, Engineering FacultyMiddle East Technical UniversityAnkaraTurkey
  3. 3.Food Engineering Department, Agriculture FacultyCukurova UniversityAdanaTurkey

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