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Valorization of Parthenium hysterophorus Biomass by its Utilization in Endoglucanase Production by Penicillium citrinum NAF5

  • Anita Saini
  • Neeraj K. AggarwalEmail author
Original Paper
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Abstract

Purpose

The primary aim of the present study was to evaluate the potential of P. hysterophorus weed biomass valorization through its utilization in the production of cellulase enzyme.

Methods

The work involved bio-prospecting of fungal microbes and selecting the most potent fungal isolate for its cellulolytic potential. Thereafter, endoglucanase production by the fungal isolate was enhanced by utilizing P. hysterophorus biomass as a substrate. The optimization study involved the production of the enzyme under both stationary as well as shaking conditions. A scale-up for the enzyme production was also carried out under batch mode of fermentation in Erlenmeyer flasks as well as in a 2 l-bioreactor.

Results

Penicillium citrinum NAF5, isolated from garden soil, was found as a potent cellulase producer. Under optimized conditions, P. citrinum NAF5 produced 4.224 ± 0.035 U/ml and 3.13 ± 0.026 U/ml endoglucanase in the stationary and shaking conditions of fermentation respectively, in the presence of steam pretreated P. hysterophorus biomass as the substrate. Scale-up in the flasks resulted in 3.86% and 12.11% increase in enzyme production under stationary and shaking conditions respectively; while 24.12% enhancement in enzyme production was achieved in a 2 l fermenter.

Conclusions

The study reveals that the biomass from P. hysterophorus can be utilized for cellulase production, and this can be an effective approach for the valorization of this weed’s biomass. Also, cellulase production by P. citrinum NAF5 fungus is appreciable and holds potential for utilization in various applications.

Keywords

Cellulase Cellulolytic Fungus Lignocellulosic biomass P. hysterophorus P. citrinum 

Notes

Compliance with Ethical Standards

Conflict of interest

The authors declare that there’s no conflict of interest regarding publication of this paper.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of MicrobiologyKurukshetra UniversityKurukshetraIndia

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