Waste and Biomass Valorization

, Volume 10, Issue 11, pp 3351–3361 | Cite as

Effect of Partial Enzymatic Hydrolysis on Physicochemical and Foaming Properties of Ovary from Squid Loligo formosana

  • Avtar Singh
  • Soottawat BenjakulEmail author
Original Paper


Squid ovary (SO) was treated with 1% Alcalase or 1% papain at different hydrolysis times and foaming properties of resulting hydrolysates were investigated. SO hydrolysate (SOH) prepared by Alcalase for 30 min (SOH-Al-30) with degree of hydrolysis (DH) of 1.79% showed the highest foaming capacity (FC) (236%). For SOH prepared by papain for 15 min with DH of 0.21%, FC of 221% was attained. Foams from both SOH were collapsed by 27% when incubated at 25 °C for 60 min. The SOH-Al-30 powder showed high solubility and surface hydrophobicity. Particle size of SOH-Al-30 powder was slightly higher than that of squid ovary powder (SOP). FTIR spectra indicated that hydrolysis slightly affected the conformation and interaction of protein molecules. SOH-Al-30 pre-heated at 60 °C showed the highest FC (300%) and had the lowest liquid drainage when whipped for 4 min. Viscoelastic properties and microstructure of foam from pre-heated SOH-Al-30 were much improved than those from SOP. Thus, enzymatic hydrolysis having the controlled DH, followed by pre-heating at optimum temperature with the appropriate whipping time could improve foaming properties of squid ovary.


Squid ovary Partial hydrolysis Alcalase Papain Foaming properties Lamella 



This research was supported by the Higher Education Research Promotion and the Thailand’s Education Hub for Southern Region of ASEAN Countries (THE-AC, 2015) scholarship and Graduate School, Prince of Songkla University. The TRF Distinguished Research Professor Grant was also acknowledged.


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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Food Technology, Faculty of Agro-IndustryPrince of Songkla UniversityHat YaiThailand

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