Microbial Induced Calcium Carbonate Precipitation (MICP) Using Pig Urine as an Alternative to Industrial Urea
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Biocalcification by microbial induced CaCO3 precipitation (MICP) (catalyzes by microbial enzyme urease) is a promising field of research in recent decades due to its versatile application in industry. Besides, the animal waste disposal has attracted much attention owing to increasing awareness of environmental protection and resource substitution. Though, the biotechnical application of Pig urine which causes of environmental pollution due to existence of high amount of urea or pig urea (PU), not study yet. Present study, PU was used instead of industrial urea, first time, as the enzymatic substance to produced CaCO3 by four sets of experimental quartz-sand column (Control, A (4 h), B (6 h) and C (12 h)) with different injection time intervals (4 h, 6 h and 12 h), bacterial (Sporosarcina pasteurii, DSM 33) amount, pig urine and with 1.1 (M) CaCl2. The results revealed the precipitation of CaCO3 from PU and CaCl2 in presence of S. pasteurii. The porosity and permeability of the quartz-sand column was noted to decrease with the application of pig urine. The mechanical properties (anti-permeability) of the quartz-sand columns were noticed better, while time interval between (4 h) the application of pig urine and bacterial solution was shorter (CaCO3 formation increase by 43%, compare to control). The XRD and SEM results displayed the CaCO3 formation, which confirmed the feasibility of PU as carbonate source. The findings implied a cost reduction of MICP technology by using pig urine as urea, animal waste mitigation for environmental pollution and resource substitution.
KeywordsMICP Pig urine Quartz-sand columns CaCO3 Resources management
Authors would like to thank Ministry of Science and Technology (Taiwan) for financial support (MOST 105-2811-M-194-014 and MOST 106-2811-M-194-006).
- 2.Anbu, P., Kang, C.H., Shin, Y.J., So, J.S.: Formations of calcium carbonate minerals by bacteria and its multiple applications. SpringerPlus 5(250), 1–26 (2016)Google Scholar
- 6.European Commission Directive 2003/30/EC: European Parliament and of the Council of 8 May 2003 on the Promotion of the Use of Biofuels or Other Renewable Fuels for Transport (2003)Google Scholar
- 8.Hariharan, M., Varghese, N., Cherian, A.B., Sreenivasan, P.V., Paul, J., Asmy Antony, K.A.: Synthesis and characterisation of CaCO3 (calcite) nano particles from cockle shells using chitosan as precursor. Int. J. Sci. Res. Publ. 4(10), 1–5 (2014)Google Scholar
- 11.Hsu, C.M., Huang, Y.H., Chen, H.J., Lee, W.C., Chiu, H.W., Maity, J.P., Chen, C.C., Kuo, Y.H., Chen, C.Y.: Green synthesis of nano-Co3O4 by microbial induced precipitation (MIP) process using Bacillus pasteurii and its application as supercapacitor. Mater. Today Commun. 14, 302–311 (2018)CrossRefGoogle Scholar
- 13.Kenpitts apes. Porosity and Permeability Lab. http://kenpitts.net/apes/earth_systems/2007/porosity_permeability_lab.htm. (2007). Accessed 05 June 2017
- 17.Ma, J.A.: Web-based Spatial Decision Support System for Utilizing Organic Wastes as Renewable Energy Resources in New York State. Ph.D. dissertation. Cornell University (2006)Google Scholar
- 18.Mujah, D., Shahin, M.A., Cheng, L.: State-of-the-art review of biocementation by microbially induced calcite precipitation (MICP) for soil stabilization geomicrobiology. Journal 34(6), 524–537 (2017)Google Scholar
- 29.Whiffin, V.S.: Microbial CaCO3 precipitation for the production of biocement. PhD Thesis, Murdoch University, Perth, WA, p162 (2004)Google Scholar