Scenedesmus Biomass Productivity and Nutrient Removal from Wet Market Wastewater, A Bio-kinetic Study
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The current study aims to investigate the production of microalgae biomass as a function for different wet market wastewater ratios (10, 25, 50, 75 and 100%) and Scenedesmus sp. initial concentrations (104, 105, 106, 107 cells/mL) through the phycoremediation process. The biomass production, total nitrogen (TN), total phosphorus (TP) and total organic compounds (TOC) were determined daily. The pseudo-first order kinetic model was used to measure the potential of Scendesmus sp. in removing nutrients while the Verhulst logistic kinetic model was used to study the growth kinetic. The study revealed that the maximum productivity of Scenedesmus sp. biomass was recorded with 106 cells/mL of the initial concentration in 50% wet market wastewater (98.54 mg/L/day), and the highest removal of TP, TN, and TOC was obtained (85, 90 and 65% respectively). Total protein and lipid contents in the biomass yield produced in the wet market wastewater were more than that in the biomass produced in the BBM (41.7 vs. 37.4 and 23.2 vs. 19.2%, respectively). The results of GC–MS confirmed detection of 44 compounds in the biomass from the wet market wastewater compared to four compounds in the BBM. These compounds have several applications in pharmaceutical and personal care products, chemical industry and antimicrobial activity. These findings indicated the applicability of wet market wastewater as a production medium for microalgae biomass.
KeywordsPseudo-first order kinetic model Verhulst logistic kinetic model Growth rate Scenedesmus sp.
This research was funded under EScience Fund Project No. (02-01-13-SF0135) provided by the Ministry of Science, Technology and Innovation, Malaysia (MOSTI).Special thanks to Dr. Hazel Monica Matias-Peralta for providing the microalgae seed and any parties involved in this project specifically Universiti Tun Hussein Onn Malaysia for providing the equipment and research facilities. This paper was also partly sponsored by the Office for Research, Innovation, Commercialization, and Consultancy Management (ORICC) under grant votU682.
- 2.Amneera, W.A., Najib, N.W.A.Z., Yusof, S.R.M., Ragunathan, S.: Water quality index of Perlis River, Malaysia. Int. J. Civ. Environ. Eng. 3(2), 1–6 (2013)Google Scholar
- 5.Atiku, H., Mohamed, R.M.S.R., Wurochekke, A.: Bathroom greywater bioremediation by microalgae Botryococcus sp.. Ind. J. Sci. Technol. 9(46), 1–6 (2016)Google Scholar
- 8.Badwy, M.T., Ibrahim, E.M., Zeinhom, M.M.: Partial replacement of fish meal with dried microalga (Chlorella spp. and Scenedesmus spp.) in Nile Tilapia (Oreochromis niloticus) diets, Int. Symposium on Tilapia in Aquaculture (2008)Google Scholar
- 10.Darmaki, A., Govindrajan, A.L., Talebi, S., Al-Rajhi, S., Al Barwnai, T., Bulashi, A.Z.: Cultivation and Characterization of Microalgae for Wastewater Treatment, vol. 1, World Congress on Engineering, London (2012)Google Scholar
- 11.Vizcaino, J., Lopez, G., Saez, M.I., Jimenez, J.A., Barros, A., Hidalgo, L., Camacho-Rodrigues, J., Martinez, T.F., Ceron-Garcia, M.C., Alarcon, F.J.: Effect of the microalga Scenedesmus almeriensisas fishmeal alternative in diets for gilthead sea bream, Sparus aurata, juveniles. Aquaculture (2014) https://doi.org/10.1016/j.aquaculture.2014.05.010 Google Scholar
- 13.Kim, M.K., Park, J.W., Park, C.S., Kim, S.J., Jeune, K.H., Chang, M.U., Acreman, J.: Enhanced production of Scenedemus spp. (green microalgae) using a new medium containing fermented swine wastewater. Bioresour. Technol. 98, 220–2228 (2007). https://doi.org/10.1016/j.biortech.2006.08.031 Google Scholar
- 14.Lu, Q., Zhou, W., Min, M., Ma, X., Chandra, C., Doan, Y.T.T., Ma, Y., Zheng, H., Cheng, S., Griffith, R., Chen, P., Chen, C., Urriola, P.E., Shurson, G.C., Gislerod, H.R., Ruan, R.: Growing Chorella sp. on meat processing wastewater for nutrient removal and biomass production. Bioresour. Technol. 198, 189–197 (2015). https://doi.org/10.1016/j.biotech.2015.08.133 CrossRefGoogle Scholar
- 15.APHA.: (2012). Standard Methods for the Examination of Water and Wastewater, American Public Health Association, AWWA (American Water Works Association, Washington, D.C.Google Scholar
- 16.Nichols, H., Bold, H.: Growth media. In: Jr, S., Hand, B. (ed.) Physiol Methods, pp. 7–24. Cambridge University Press, Cambridge (1965)Google Scholar
- 17.Ruiz, J., Arbib, Z., Alvarez-Diaz, P., Gariddo-Perez, C., Barragan, J., Perales, J.A.: Photobiotreatment model (PhBT): a kinetic model for microalgae biomass growth and nutrient removal in wastewater. Environ. Technol. 34, 979–991 (2013). https://doi.org/10.1080/0959330.2012.724451 CrossRefGoogle Scholar
- 21.Arbib, Z., Ruiz, J., Alvarez-Diaz, P., Gariddo-Perez, C., Perates, J.A.: Capability of different microalgae species for phytoremediation processes: wastewater tertiary treatment, CO2 bio-fixation and low cost biofuels production. Water Res. 49, 465–474 (2014). https://doi.org/10.1016/j.watres.2013.10.036 CrossRefGoogle Scholar
- 22.Mamun, A., Amid, A., AbdKarim, I., Alam, Z., Abu Sayem, S.M.: Phytoremediation and mathematical model for domestic wastewater by Chlorella vulgaris. Adv. Environ. Biol. 9, 139–144 (2015)Google Scholar
- 23.A.O.A.C Association of Official Analytical Chemists.: Official Methods of Analysis, 18th edn., Association of Official Analytical Chemists, Washington, D.C. (2005)Google Scholar
- 25.Folch, A.L.S.: A simple method for isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509 (1957)Google Scholar
- 27.Zulkifli, A.R., Roshadah, H., TunkuKhalkausar, T.F.: Control of Water Pollution from Nonindustrial Premises. Department of Environment, Putrajaya (2011)Google Scholar
- 34.Shen, Q.H., Jiang, J.W., Chen, L.P., Cheng, L.H., Xu, X.H., Chen, H.L.: Effect of carbon source on biomass growth and nutrients removal of Scenedesmusobliquus for wastewater advanced treatment and lipid production, Bioresour. Technol. (2015), https://doi.org/10.1016/j.biortech.2015.04.053 Google Scholar