Waste and Biomass Valorization

, Volume 10, Issue 10, pp 2959–2965 | Cite as

Utilization of Cuttlefish Liver Waste for Oil Production: Evaluation of Quality Characteristics and Biological Activity

  • Meivelu MoovendhanEmail author
  • S. Vairamani
  • A. Shanmugam
Original Paper


The ash, moisture, protein and carbohydrate content of the extracted oil were estimated as 4, 7, 4 and 1% respectively. The Vitamins A and E was found to be 59.14 and 17.4 mg/100 g. Nine fatty acids were found and the Linoleic acid, DHA and EPA were recorded as higher. The specific gravity, solidification point, refractive index, saponification value, iodine value and acid value were also calculated and found as 1.056, 1.2 °C, 1.465 µ, 50.5, 20.4 mg and 0.075 mg KOH/g. The antioxidant activity that includes total antioxidant activity, DPPH & Hydroxyl radical scavenging assay, reducing power and ferrous ion chelating activity of liver oil was studied by following standard methods and results of all the assays was found increasing with increase in concentration. Further purification of liver oil and studying its antioxidant property in animal model shall form the basis for its use as a potent alternate natural antioxidant and nutrition supplements.


Sepia pharonis Liver oil Physical property Antioxidant activity 



Authors are thankful to the Dean and Director, CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University for providing all necessary facilities. The authors are also thankful to the Centre for Marine Living Resources and Ecology (CMLRE), Ministry of Earth Sciences, Kochi for the financial assistance.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.


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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centre of Advanced Study in Marine Biology, Faculty of Marine SciencesAnnamalai UniversityParangipettaiIndia
  2. 2.Bioengineering and Drug Design Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of BiosciencesIndian Institute of Technology, Madras (IIT-M)ChennaiIndia

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