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Waste and Biomass Valorization

, Volume 10, Issue 9, pp 2651–2659 | Cite as

Cellulase Production from Pre-treated Pea Hulls Using Trichoderma reesei Under Submerged Fermentation

  • Ranjna SirohiEmail author
  • Anupama Singh
  • Ayon Tarafdar
  • Navin Chandra Shahi
  • Ashok Kumar Verma
  • Anurag Kushwaha
Original Paper

Abstract

Purpose

Pea hulls have a significant contribution to agricultural waste and are left unattended. It has a rich nutrient media and may have application in waste utilization. In this investigation, the potential of pea hulls for the production of cellulase has been explored.

Methods

The effect of H2O2 concentration, agitation speed and harvesting time on cellulase production by Trichoderma reesei QM9414 under submerged fermentation at constant pH of 5.0 and temperature 30 ± 0.1 °C were studied.

Results

The maximum filter paper (FP) cellulase activity of 0.372 ± 0.019 U/mL was obtained at 91 h incubation period and 120 rpm agitation speed. Based on the optimized results of fermentation parameters, 120 rpm agitation speed, 5% H2O2 concentration and 91 h harvesting time was recommended for efficient cellulase production. The effect of harvesting time on protein, reducing sugar and cellulase activity was pre-dominant. The purified cellulase enzyme specific activity was recorded as 13.8 U/mL.

Conclusions

The results indicate that the production of cellulase from green pea hulls may provide a novel and economical solution for industrial waste disposal.

Keywords

Cellulase Pea hull Submerged fermentation Trichoderma reesei 

List of symbols

β0, βi, βij, βii

Model coefficients

p

Number of explanatory variables (excluding constants)

yi

Observed data

\({\bar {y}_l}\)

Mean of observed data

\({\hat {y}_l}\)

Predicted data

N

Sample size

Xi,j

Independent variables

Y

Dependent variable

Notes

Acknowledgements

This work was submitted as thesis in partial fulfillment for the requirement of the degree of Master of Technology of the first author. The authors thankfully acknowledge the financial assistance received under University funded project, G. B. Pant University of Agriculture & Technology, Pantnagar. We extend our gratitude to all the colleagues of the department for helping us in the manual separation of pea from pea pods.

References

  1. 1.
    Rashad, M.M., Nooman, M.U.: Production, purification and characterization of extracellular invertase from Saccharomyses Cerevisiae NRRL Y- 12632 by solid-state fermentation of red carrot residue. Aus. J. Basic and Appl. Sci. 3(3), 1910–1919 (2009)Google Scholar
  2. 2.
    Arrigoni, E., Caprez, A., Amado, R., Neukom, H.: Food hydrocolloids. Food Biochem. 1, 57–64 (1986)Google Scholar
  3. 3.
    Ralet, M.C., Valle, D.G., Thibault, J.F.: Raw and extruded fiber from pea hulls. I. Composition and physico-chemical properties. Carbohydr. Polym. 20, 17–23 (1993)CrossRefGoogle Scholar
  4. 4.
    Sosulski, F.W., Wu, K.K.: High-fiber breads containing field pea hulls, wheat, corn, and wild oat brans. Cereal Chem. 65(3), 186–191 (1988)Google Scholar
  5. 5.
    Gao, J.: Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid state cultivation of corn stover. Biores. Technol. 99, 3634–3637 (2008)Google Scholar
  6. 6.
    Mushimiyimana, I., Tallapragada, P.: Agro wastes residues as strategy to produce cellulase. Int. J. Chem.Tech. Res. 8(1), 89–97 (2015)Google Scholar
  7. 7.
    El-Shishtawy, R.M., Mohamed, S.A., Asiri, A.M., Gomaa, A.M., Ibrahim, H.I., Tahhi, A.H.: Saccharification and hydrolytic enzyme production of alkali pre-treated wheat bran by Trichoderma virens under solid state fermentation. BMC Biotechnol. 15, 37 (2015)CrossRefGoogle Scholar
  8. 8.
    Muthuvelayudham, R., Viruthagiri, T.: Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw. Afr. J. Biotechnol. 5(20), 1873–1881 (2006)Google Scholar
  9. 9.
    Fatima, G., Satinder, K.B., Tyagi, R.D., Verma, M., Surampalli, R.Y.: Screening of agro-industrial wastes to produce ligninolytic enzymes by Phanerochaete chrysosporium. Biochem. Eng. J. 49, 388–394 (2010)CrossRefGoogle Scholar
  10. 10.
    Esfahani Hamidi, Z., Salimi Rocky, K.: Evaluation of the effect of particle size, aeration rate and harvest time on the production of cellulase by Trichoderma reesei QM9414 using response surface methodology. Food Bioprod. Process. 88, 61–66 (2010)CrossRefGoogle Scholar
  11. 11.
    Verma, N., Bansal, C., Mukesh Kumar, V.: Pea peel waste: a lignocellulosic waste and its utility in cellulase production by Trichoderma reesei under solid state cultivation. Bio Resour. 6(2), 1505–1519 (2011)Google Scholar
  12. 12.
    Kang, S.W., Park, Y.S., Lee, J.S., Hong, S.I., Kim, S.W.: Production of cellulases and hemicellulases by A.niger KK2 from lignocellulosic biomass. Biores. Technol. 91, 153–156 (2004)CrossRefGoogle Scholar
  13. 13.
    Javed, I.M.M., Khant, T.S.: An innovative approach for hyperproduction of cellulolytic and hemicellulolytic enzymes by consortium of Aspergillus niger MSK-7 and Trichoderma viride MSK-10. Afr. J. Biotechnol. 5(8), 609–614 (2006)Google Scholar
  14. 14.
    Dhillon, G.S., Oberoi, H.S., Kaur, S., Bansal, S., Brar, S.K.: Value-addition of agricultural wastes for augmented cellulase and xylanase production through solid-state tray fermentation employing mixed-culture of fungi. Ind. Crops Prod. 34, 1160–1167 (2011)CrossRefGoogle Scholar
  15. 15.
    Damato, G., Vivona, G., Stoller, M., Bubbico, R., Bravi, M.: Cellulase production from olive processing residues. Chem. Eng. Trans. 20, 978–988 (2010)Google Scholar
  16. 16.
    Saravanan, P., Muthuvelayudham, R., Rajesh, K.R., Viruthagiri, T.: Optimization of cellulase production using Trichoderma reesei by RSM and comparison with genetic algorithm. Front. Chem. Sci. Eng. 6(4), 443–452 (2012)CrossRefGoogle Scholar
  17. 17.
    Sukumaran, R.K., Singhania, R.R., Mathew, G.M., Pandey, A.: Cellulase production using biomass feedstock and its application in lignocellulose saccharification for bio-ethanol production. Renew. Energy. 34(2), 421–424 (2006)CrossRefGoogle Scholar
  18. 18.
    Moosavi-Nasab, M., Majdi-Nasab, M.: Cellulase production by Trichoderma reesei using sugar beet pulp. Iran Agri. Res. 25(1–2), 107–116 (2007)Google Scholar
  19. 19.
    Selvakumar, G., Saha, S., Kundu, S.: Inhibitory activity of pine needle extracts on some agriculturally resourceful microbes. Ind. J. Microbiol. 47(3), 267–270 (2007)CrossRefGoogle Scholar
  20. 20.
    Miller, G.L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426–428 (1959)CrossRefGoogle Scholar
  21. 21.
    Bhavna, M.V., Magar, J.G.: Use of agricultural wastes for cellulases production by Aspergillus niger with submerged and solid state fermentation. Bionano Front. 3, 189–192 (2010)Google Scholar
  22. 22.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)Google Scholar
  23. 23.
    Ghose, T.K.: Measurements of cellulase activities. Pure Appl. Chem. 59, 257–268 (1987)CrossRefGoogle Scholar
  24. 24.
    Singhania, R.R., Sukumaran, R.K., Patel, A.K., Larroche, C., Pandey, A.: Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzyme Microb. Tech. 46, 541–549 (2010)CrossRefGoogle Scholar
  25. 25.
    Jhadhav, A.R., Girde, A.V., More, S.M., More, S.B., Khan, S.: Cellulase production by utilizing agriculture wastes. Res. J. Agri. For. Sci. 1(7), 6–9 (2013)Google Scholar
  26. 26.
    Devi, M.C., Kumar, M.S.: Production, Optimization and partial purification of cellulase by Aspergillus niger fermented with paper and timber sawmill industrial wastes. J. Microbiol. Biotechnol. Res. 2, 120–128 (2012)Google Scholar
  27. 27.
    Nema, N., Alamir, L., Mohammad, M.: Production of cellulase from Bacillus cereus by submerged fermentation using corn husks as substrates. Int. Food Res. J. 22, 1831–1836 (2015)Google Scholar
  28. 28.
    Mrudula, S., Murugammal, R.: Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate. Braz. J. Microbiol. 42, 1119–1127 (2011)CrossRefGoogle Scholar
  29. 29.
    Tallapragada, P., Venkatesh, K.: : Isolation, identification and optimization of xylanase enzyme produced by aspergillus niger under submerged fermentation. J. Microbiol. Biotechnol. Res. 1, 137–147 (2011)Google Scholar
  30. 30.
    Shen, D., Xiao, R., Gu, S., Luo, K.: The pyrolytic behavior of cellulose in lignocellulosic biomass: a review. RSC Adv. 1, 1641–1660 (2011)CrossRefGoogle Scholar
  31. 31.
    Zhang, P.Y.H., Himmel, E., Michael, M.R.J.: Outlook for cellulase improvement: screening and selection strategies. Biotechnol. Adv. 24, 452–481 (2006)CrossRefGoogle Scholar
  32. 32.
    Rabelo, S.C., Carrere, H., Filno, M.R., Costa, A.C.: Production of bioethanol, methane and heat from sugarcane bagasse in a biorefinery concept. Biores. Technol. 102, 7887–7895 (2011)CrossRefGoogle Scholar
  33. 33.
    John, R.P., Nampoothiri, M., Pandey, A.: Solid-state fermentation for l-lactic acid production from agro wastes using Lactobacillus delbrueckii. Process Biochem. 41, 759–763 (2006)CrossRefGoogle Scholar
  34. 34.
    Wang, Q., Wang, X., Wang, X., Ma, H.: Glucoamylase production from food waste by Aspergillus niger under submerged fermentation. Process Biochem. 43, 280–286 (2008)CrossRefGoogle Scholar
  35. 35.
    Raimbault, M.: Solid State Fermentation: Growth Filamentous Fungi on Starch Substrate, pp. 127–291. ORSTOM, Paris (1981)Google Scholar
  36. 36.
    Santhanam, A., Yalentesfa, B., Alemu, T.: Solid substrate fermentation and conversion of orange waste into fungal biomass using Aspergillus niger KA-06 and Chaetomium Spp KC-06. Afr. J. Microbiol. Res. 4(12), 1275–1281 (2010)Google Scholar
  37. 37.
    Ghosh, P., Ghosh, U.: Statistical optimization of laccase production by Aspergillus flavus PUF5 through submerged fermentation using agro-waste as cheap substrate. Acta Biol. Szeged. 61, 25–33 (2017)Google Scholar
  38. 38.
    Fratebianchi, D., Crespo, J.M., Tari, C., Cavalitto, S.: Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes. J. Chem. Technol. Biotechnol. 92, 305–310 (2016)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Ranjna Sirohi
    • 1
    Email author
  • Anupama Singh
    • 1
  • Ayon Tarafdar
    • 1
  • Navin Chandra Shahi
    • 1
  • Ashok Kumar Verma
    • 2
  • Anurag Kushwaha
    • 1
  1. 1.Department of Post Harvest Process and Food Engineering, College of TechnologyG.B. Pant University of Agriculture and TechnologyPantnagarIndia
  2. 2.Department of Biochemistry, College of Basic Sciences and HumanitiesG.B. Pant University of Agriculture and TechnologyPantnagarIndia

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