Enzymatic Conversion of Glycerol to 2,3-Butanediol and Acetoin by Serratia proteamaculans SRWQ1
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Biodiesel, a renewable and environment friendly biofuel is produced by transesterification process using animal fats and vegetable oils. However, the flourishing of biodiesel industries has led to produce a huge amount (10% v/v) of crude glycerol as a core by-product, created an overflow problem. Therefore, biotransformation of glycerol into biofuel and other value-added products is one of the promising applications of glycerol due to its high availability at low cost. In this study, we report the capability of converting glycerol as a sole carbon source to 2,3-butanediol (2,3-BDO) and acetoin by using a newly isolated Serratia proteamaculans SRWQ1 strain in batch biotransformation process under aerobic condition. Strain SRWQ1 displayed a maximum up to 18.43 ± 1.55 g/L of 2,3-BDO, yielding 0.4 g/g using 49.0 g/L glycerol which was 98.0% of glycerol utilization. The strain SRWQ1 also successfully produced a significant amount of acetoin 8.38 ± 0.76 g/L with yields 0.06 g/g. Moreover, the maximum activity of glycerol dehydrogenase (GDH) which is a key enzyme of glycerol metabolisms was 408.69 ± 0.069 units/mg protein. The newly isolated strain S. proteamaculans SRWQ1 displayed the best ability to synthesize 2,3-BDO and acetoin using glycerol as the sole substrate, and it is the first report on biotransformation of glycerol by S. proteamaculans. Therefore, this aerobic conversion of glycerol to value-added green products 2,3-BDO and acetoin with potential industrial applications would represent a noteworthy alternative to add value for biodiesel production helping biodiesel industries development.
KeywordsGlycerol dehydrogenase Bioconversion Serratia proteamaculans 2,3-Butanediol Acetoin
This work was financially supported by NSERC-RDF to W. Q. and Saudi Ministry of Higher Education (Award No. KAS8020509) of Saudi Arabia to I. A.
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