Waste and Biomass Valorization

, Volume 10, Issue 4, pp 755–762 | Cite as

Enzymatic Degradation of the Rice Bran: Problem or Opportunity?

  • Evandro Pereira
  • Sandra Einloft
  • Marcus Seferin
  • Leonardo Moreira dos Santos
  • Jeane Lima
  • Rosane LigabueEmail author
Original Paper


This study shows rice bran as feedstock with high potential for biodiesel production by enzyme catalysis in situ, due their unique characteristics that allow to control its composition (triglycerides and free fatty acids). Among the characteristics evaluated of rice bran (particle size, moisture and metals concentration), the particle size significantly influenced the hydrolysis of triglycerides into free fatty acids. The oil content obtained from extraction of different granulometric ranges of rice bran (dry basis, 9 h) was 43.6% (20–32 mesh), 28.2% (32–35 mesh), 25.8% (35–60 mesh) and 24.8% (60–100 mesh). The presence of trace elements Zn, Cu and Ca in larger concentration in the range of 20–32 mesh may explain the greater amount of oil in this fraction, because these metals act as inhibitors of the enzyme activity in the triglyceride degradation. Free fatty acids formation rate was around 0.73% day−1 in the degradation conditions studied (10.5% moisture and 6 weeks).


Rice bran Lipase Enzymatic activity Triglycerides hydrolysis 



The authors would like to thank CAPES and FAPERGS for fellowships, FINEP (project 2520/2009), PUCRS and FAPERGS-PRONEX for partial financial support. IPR/PUCRS for ICP-OES analyzes.


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Evandro Pereira
    • 1
  • Sandra Einloft
    • 1
    • 2
  • Marcus Seferin
    • 1
    • 2
  • Leonardo Moreira dos Santos
    • 1
  • Jeane Lima
    • 2
    • 3
  • Rosane Ligabue
    • 1
    • 2
    Email author
  1. 1.Graduation Program in Materials Engineering and TechnologyPontifical Catholic University of Rio Grande do SulPorto AlegreBrazil
  2. 2.School of ChemistryPontifical Catholic University of Rio Grande do SulPorto AlegreBrazil
  3. 3.Institute of Petroleum and Natural ResourcesPontifical Catholic University of Rio Grande do SulPorto AlegreBrazil

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