Waste and Biomass Valorization

, Volume 10, Issue 5, pp 1295–1302 | Cite as

Towards a Sustainable Route for the Production of Squalene Using Cyanobacteria

  • Mariane Bittencourt Fagundes
  • Raquel Guidetti Vendruscolo
  • Mariana Manzoni Maroneze
  • Juliano Smanioto Barin
  • Cristiano Ragagnin de Menezes
  • Leila Queiroz Zepka
  • Eduardo Jacob-Lopes
  • Roger WagnerEmail author
Original Paper


Treatment of slaughterhouse wastewater is a huge industrial problem. The use of an algae biorefinery platform could be a sustainable technological alternative that produces value-added compounds instead of dumping the wastewater. For this reason, this research aimed to evaluate squalene production from the microalgae Phormidium autumnale cultivated using agroindustrial wastewater. A derivatization method was performed to determine the squalene and fatty acids content, evaluated by gas chromatography with flame ionization and mass spectrometry detectors. A total of 0.18 g/kg of squalene were found in the biomass, with a high content of unsaturated fatty acids (52%). Sensitivity analysis estimated production of 727–72,750 kg/year in industries with different capacities. In this sense, P. autumnale in agroindustrial wastewater could offer a potential alternative method of squalene production.

Graphical Abstract


Bioactive compound Gas chromatography Microalgae Phormidium autumnale Sensitivity analysis 



The present study was carried out with the financial support of the National Council for Scientific and Technological Development (CNPq)- Brazil and funding from FAPERGS - Foundation for Research Support of the State of Rio Grande do Sul, Porto Alegre, RS, Brazil and CAPES improving coordination of Higher Education Personnel for supporting this research.

Compliance with Ethical Standards

Conflict of interest

Mariane Bittencourt Fagundes declares that she has no conflict of interest and all the other authors: Raquel Guidetti Vendruscolo, Mariana Manzone Maroneze, Cristiano Ragagnin Menezes, Leila Queiroz Zepka, Juliano Smanioto Barin, Eduardo Jacob-Lopes and Roger Wagner also declares that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Mariane Bittencourt Fagundes
    • 1
  • Raquel Guidetti Vendruscolo
    • 1
  • Mariana Manzoni Maroneze
    • 1
  • Juliano Smanioto Barin
    • 1
  • Cristiano Ragagnin de Menezes
    • 1
  • Leila Queiroz Zepka
    • 1
  • Eduardo Jacob-Lopes
    • 1
  • Roger Wagner
    • 1
    Email author
  1. 1.Department of Food Science and TechnologyFederal University of Santa MariaSanta MariaBrazil

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