Waste and Biomass Valorization

, Volume 10, Issue 5, pp 1267–1276 | Cite as

Physic Nut Seed Cake Methanation and Chemical Characterization of Anaerobic Bio-digested Substrate

  • Freddy Zambrano Gavilanes
  • Carmen Luísa Barbosa Guedes
  • Helder Rodrigues Silva
  • Rafael Guayato Nomura
  • Diva Souza AndradeEmail author
Original Paper


Methane (CH4) generation from the anaerobic digestion of vegetal biomass is an important clean alternative form of energy. In this context, the response of the physic nut seed cake to microbial inoculum for biogas and CH4 production was examined in lab-scale biogas anaerobic digesters. Two experiments were carried out with physic nut seed cake stored over 6 and 18 months. The treatments assayed in each one of these experiments included five types of inoculum: water as the control, three different consortia of bacterial isolates from Jatropha seed cake and one bacterial consortium from bio-digested swine manure. The cake stored for 18 months with water showed the highest volume of biogas, with 83.67 mL in 6.54 g of cake after 21 days, but with lower production of CH4. The best concentration of CH4 (84.33%) was obtained from the Jatropha seed cake stored for 18 months when co-digested with the consortium of 14 bacterial isolates. Co-digestion of physic nut seed cake with the bacterial consortium of isolates from Jatropha cake was the best for producing CH4. After anaerobic digestion, substrates had an increase in macro and micronutrient contents, showing potential for biofertilizer production.

Graphical Abstract


Bacterial consortium Biogas Biofertilizer Jatropha curcas Methane 



The authors are grateful to Proyecto Piñón para Galápagos-Ecuador for kindly supply Jatropha seed cake. H.R.S. and D.S.A acknowledge PhD fellowship from CAPES (National Council for the Improvement of Higher Education) and a grant from Fundação Araucária/Paraná, Brazil, respectively.

Compliance with Ethical Standards

Conflict of interest

The authors declare no competing financial interest.


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Freddy Zambrano Gavilanes
    • 1
    • 2
  • Carmen Luísa Barbosa Guedes
    • 3
  • Helder Rodrigues Silva
    • 1
    • 2
  • Rafael Guayato Nomura
    • 2
  • Diva Souza Andrade
    • 2
    Email author
  1. 1.Agronomy DepartmentState University of Londrina (UEL)LondrinaBrazil
  2. 2.Agricultural Research Institute of Paraná State (IAPAR)LondrinaBrazil
  3. 3.Chemistry Department, CCEState University of Londrina (UEL)LondrinaBrazil

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