Waste and Biomass Valorization

, Volume 10, Issue 4, pp 1053–1064 | Cite as

Effect of SDS and Neutral Protease on the Release of Extracellular Polymeric Substances (EPS) from Mechanical Dewatered Sludge

  • Weiyun WangEmail author
  • Xuemei Gao
  • Jin Zhang
  • Tianhua Yang
  • Rundong Li
  • Yang Sun
Original Paper


Extracellular Polymeric Substances (EPS) are complex high-molecular-weight secretions from microorganisms produced by cell lysis and adsorbed organic matter in wastewater that has crucial effects on sludge dewaterability. In this study, the effects of neutral protease and sodium dodecyl sulfate (SDS) on the amount and characteristics of EPS and on the moisture distribution in mechanical dewatered sludge were observed. The treatment was effective, and the content of EPS increased by 241% after treatment with 0.15 g/g total suspended solid (TSS) SDS and 0.03 g/g TSS neutral protease. Moreover, the ratio of PN/PS increased from 5.76 (blank) to 7.81 (treated with 0.10 g/g TSS SDS and 0.03 g/g TSS neutral protease). The results also showed that the content of bound water decreased from 7.40 to 6.29 g/g TSS in response to the addition of 0.15 g/g TSS SDS and 0.03 g/g TSS neutral protease, and the percentage of moisture with a lower binding force increased from 96.07 to 97.60% after treatment, which indicated that the dewaterability of sludge improved after treatment. Furthermore, 3D-EEM analysis indicated that the aromatic proteins and soluble microbial by-product-like materials were dominant fluorescence materials in EPS, and the content of simple aromatic proteins such as tyrosine and tryptophan in LB-EPS decreased after the addition of neutral protease and SDS, while the fluorescence materials in S-EPS and TB-EPS induced no obvious change between the original and treated sludge.


Sludge Neutral protease SDS EPS 3D-EEM 



This work was funded by the National Natural Science Foundation of China (No. 51308349) and the National Natural Science Foundation of China (No. 51276119).


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.College of Energy and EnvironmentShenyang Aerospace UniversityShenyangChina
  2. 2.Key Laboratory of Clean Energy, Liaoning ProvinceShenyangChina

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