Fishmeal Wastewater as A Low-Cost Nitrogen Source for γ-Polyglutamic Acid Production Using Bacillus subtilis
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Fishmeal wastewater, a fishmeal processing waste, was used as culture medium to study the effect of Bacillus subtilis A3 on the production of γ-polyglutamic acid (γ-PGA). The results showed that the optimum concentration of chemical oxygen demand (CODCr) for fishmeal wastewater was 15 g/L. Moreover, addition of 30 g/L glucose and 25 g/L glutamic acid in the medium was beneficial to cell growth and production of γ-PGA. The study also showed that the high salinity of wastewater had little effect on cell growth and production of γ-PGA after dilution. Thus, the optimal medium consisted of COD 15, 30 g/L glucose, 25 g/L glutamic acid, in which the average yield of γ-PGA (25.07 ± 0.34 g/L) was obtained. The study suggested that fishmeal wastewater can be a replacement for nitrogen source for γ-PGA production, and hence it can be the cost-effective alternative in γ-PGA production. Meanwhile, the process can offset the disposal costs of the wastes.
KeywordsBacillus subtilis Fishmeal wastewater Nitrogen source γ-Polyglutamic acid
This research was financially supported by State Key Laboratory of Microbial Technology (M2012-14), Shandong University.
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