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Waste and Biomass Valorization

, Volume 10, Issue 3, pp 709–720 | Cite as

Evaluation of Sludge Palm Oil as Feedstock and Development of Efficient Method for its Utilization to Produce Polyhydroxyalkanoate

  • Letchimanan Thinagaran
  • Kumar SudeshEmail author
Original Paper
  • 180 Downloads

Abstract

Purpose

Sludge palm oil (SPO), a difficult-to-be-used solid byproduct of the palm oil milling industry, was evaluated as potential carbon source for poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] production using recombinant Cupriavidus necator Re2058/pCB113.

Methods

The biosynthesis of polyhydroxyalkanoate (PHA) was conducted using single stage shake flask growth to study different parameters affecting the bacterial growth. Fed-batch fermentations were conducted using SPO to increase the PHA productivity and to reach high density culture which is deemed necessary for large scale production.

Results

Initial shake flask studies showed that SPO can be utilized by the bacteria for growth and PHA accumulation. However, the yield of SPO conversion into cell biomass and PHA was low due to the difficulty in using the solid oil in liquid medium. With the aid of surfactant and mixing strategy, SPO which consisted mainly of free fatty acids was successfully emulsified in the mineral medium and was used for cell growth and PHA accumulation whereby 10 g/L of SPO supplied as emulsion solution produced 9.7 g/L of CDW containing 74 wt% P(3HB-co-22 mol% 3HHx). The high yield of biomass obtained indicates that SPO is an excellent feedstock for this strain. Fed-batch fermentation was conducted to increase the yield and productivity whereby a biomass productivity of 1.9 g/L/h and PHA productivity of 1.1 g/L/h were achieved.

Conclusion

These results suggest that SPO, an inexpensive waste material, can be used to produce PHA in large scale for commercialization purpose with reduced production cost.

Graphical Abstract

Keywords

Polyhydroxyalkanoates Copolymer P(3HB-co-3HHx) Sludge palm oil Solid fat Cupriavidus necator 

Notes

Acknowledgements

This study was supported by APEX Delivering Excellence Grant 2012 (Grant No.: 1002/PBIOLOGI/910322) provided by Universiti Sains Malaysia. Thinagaran Letchimanan thanks the Malaysian Public Service Department for financial support. The authors thank Professor Anthony Sinskey for providing the recombinant strain used in this study.

References

  1. 1.
    Anderson, A.J., Dawes, E.A.: Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev. 54, 450–472 (1990)Google Scholar
  2. 2.
    Doi, Y.: Microbial polyesters. VCH Publishers, New York (1990)Google Scholar
  3. 3.
    Sudesh, K., Abe, H., Doi, Y.: Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog Polym Sci. 25, 1503–1555 (2000)CrossRefGoogle Scholar
  4. 4.
    Shimamura, E., Kasuya, K., Kobayashi, G., Shiotani, T., Shima, Y., Doi, Y.: Physical properties and biodegradability of microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 27, 878–880 (1994)CrossRefGoogle Scholar
  5. 5.
    Doi, Y., Kitamura, S., Abe, H.: Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 28, 4822–4828 (1995)CrossRefGoogle Scholar
  6. 6.
    Noda, I., Green, P.R., Satkowski, M.M., Schechtman, L.A.: Preparation and properties of a novel class of polyhydroxyalkanoate copolymers. Biomacromolecules 6, 580–586 (2005)CrossRefGoogle Scholar
  7. 7.
    Kaneka: Full-scale development of the world’s first completely bio-based polymer with soft and heat-resistant properties. http://www.kaneka.co.jp/kaneka-e/news/index.php?c=topics_view&pk=1396245620 (2009). Accessed 11 Jan 2017
  8. 8.
    Akiyama, M., Tsuge, T., Doi, Y.: Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermentation. Polym Degrad Stabil. 80, 183–194 (2003)CrossRefGoogle Scholar
  9. 9.
    Carter, C., Finley, W., Fry, J., Jackson, D., Willis, L.: Palm oil markets and future supply. Eur J Lipid Sci Technol. 109, 307–314 (2007)CrossRefGoogle Scholar
  10. 10.
    Malaysian Palm Oil Board: Monthly crude palm oil yield (tonnes/hectares). http://bepi.mpob.gov.my/index.php/en/statistics/yield/172-yield-2016/766-yield-2016.html (2016). Accessed 11 Jan 2017
  11. 11.
    Loo, C.-Y., Lee, W.-H., Tsuge, T., Doi, Y., Sudesh, K.: Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from palm oil products in a Wautersia eutropha mutant. Biotechnol Lett. 27, 1405–1410 (2005)CrossRefGoogle Scholar
  12. 12.
    Lee, W.H., Loo, C.Y., Nomura, C.T., Sudesh, K.: Biosynthesis of polyhydroxyalkanoate copolymers from mixtures of plant oils and 3-hydroxyvalerate precursors. Bioresour Technol. 99, 6844–6851 (2008)CrossRefGoogle Scholar
  13. 13.
    Sudesh, K., Bhubalan, K., Chuah, J.A., Kek, Y.K., Kamilah, H., Sridewi, N., Lee, Y.F.: Synthesis of polyhydroxyalkanoate from palm oil and some new applications. Appl Microbiol Biotechnol. 89, 1373–1386 (2011)CrossRefGoogle Scholar
  14. 14.
    Wong, Y.-M., Brigham, C.J., Rha, C., Sinskey, A.J., Sudesh, K.: Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator. Bioresource Technol. 121, 320–327 (2012)CrossRefGoogle Scholar
  15. 15.
    Riedel, S.L., Bader, J., Brigham, C.J., Budde, C.F., Yusof, Z.A.M., Rha, C., Sinskey, A.J.: Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations. Biotechnol Bioeng. 109, 74–83 (2012)CrossRefGoogle Scholar
  16. 16.
    Choi, J.I., Lee, S.Y.: Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. Bioprocess Eng. 17, 335–342 (1997)CrossRefGoogle Scholar
  17. 17.
    Koller, M., Salerno, A., Dias, M., Reiterer, A., Braunegg, G.: Modern biotechnological polymer synthesis: a review. Food Technol Biotechnol. 48, 255–269 (2010)Google Scholar
  18. 18.
    Cavalheiro, J.M.B.T., de Almeida, M.C.M.D., Grandfils, C., Da Fonseca, M.M.R.: Poly(3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Process Biochem. 44, 509–515 (2009)CrossRefGoogle Scholar
  19. 19.
    Verlinden, R.A., Hill, D.J., Kenward, M.A., Williams, C.D., Piotrowska-Seget, Z., Radecka, I.K.: Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator. AMB Express. 1, 1 (2011)CrossRefGoogle Scholar
  20. 20.
    Kamilah, H., Tsuge, T., Yang, T.A., Sudesh, K.: Waste cooking oil as substrate for biosynthesis of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate): Turning waste into a value-added product. Malays J Microbiol. 9, 51–59 (2013)Google Scholar
  21. 21.
    Obruca, S., Petrik, S., Benesova, P., Svoboda, Z., Eremka, L., Marova, I.: Utilization of oil extracted from spent coffee grounds for sustainable production of polyhydroxyalkanoates. Appl Microbiol Biotechnol. 98, 5883–5890 (2014)CrossRefGoogle Scholar
  22. 22.
    Riedel, S.L., Jahns, S., Koenig, S., Bock, M.C., Brigham, C.J., Bader, J., Stahl, U.: Polyhydroxyalkanoates production with Ralstonia eutropha from low quality waste animal fats. J Biotechnol. 214, 119–127 (2015)CrossRefGoogle Scholar
  23. 23.
    Ainie, K., Siew, W.L., Tan, Y.A., Ma, A.N.: Characterization of a by-product of palm oil milling. Elaeis. 7, 162–170 (1995)Google Scholar
  24. 24.
    Wafti, N.A., Nang, H.L.L., May, C.Y.: Value-added products from palm sludge oil. J Appl Sci. 12, 1199 (2012)CrossRefGoogle Scholar
  25. 25.
    Hayyan, A., Hashim, M.A., Mirghani, M.E.S., Hayyan, M., AlNashef, I.M.: Esterification of sludge palm oil using trifluoromethanesulfonic acid for preparation of biodiesel fuel. Korean J Chem Eng. 30, 1229–1234 (2013)CrossRefGoogle Scholar
  26. 26.
    Nasaruddin, R.R., Alam, M.Z., Jami, M.S.: Evaluation of solvent system for the enzymatic synthesis of ethanol-based biodiesel from sludge palm oil (SPO). Bioresource Technol. 154, 155–161 (2014)CrossRefGoogle Scholar
  27. 27.
    Nasaruddin, R.R., Alam, M.Z., Jami, M.S., Salihu, A.: Statistical optimization of ethanol-based biodiesel production from sludge palm oil using locally produced Candida cylindracea lipase. Waste Biomass Valoriz. 7, 87–95 (2016)CrossRefGoogle Scholar
  28. 28.
    Nawawi, W.M.F.W., Jamal, P., Alam, M.Z.: Utilization of sludge palm oil as a novel substrate for biosurfactant production. Bioresour Technol. 101, 9241–9247 (2010)CrossRefGoogle Scholar
  29. 29.
    Budde, C.F., Riedel, S.L., Willis, L.B., Rha, C., Sinskey, A.J.: Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl Environ Microb. 77, 2847–2854 (2011)CrossRefGoogle Scholar
  30. 30.
    Nassu, R.T., Guaraldo Gonçalves, L.A.: Determination of melting point of vegetable oils and fats by differential scanning calorimetry (DSC) technique. Grasas Aceites. 50, 16–21 (1999)CrossRefGoogle Scholar
  31. 31.
    Kjeldahl, J.: Neue methode zur bestimmung des stickstoffs in organischen körpern. Fresen J Anal Chem. 22, 366–382 (1883)CrossRefGoogle Scholar
  32. 32.
    Folch, J., Lees, M., Sloane-Stanley, G.: A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509 (1957)Google Scholar
  33. 33.
    Budde, C.F., Mahan, A.E., Lu, J., Rha, C., Sinskey, A.J.: Roles of multiple acetoacetyl coenzyme A reductases in polyhydroxybutyrate biosynthesis in Ralstonia eutropha H16. J Bacteriol. 192, 5319–5328 (2010)CrossRefGoogle Scholar
  34. 34.
    Braunegg, G., Sonnleitner, B.Y., Lafferty, R.: A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur J Appl Microbiol. 6, 29–37 (1978)CrossRefGoogle Scholar
  35. 35.
    Malaysian Palm Oil Board: Malaysian Palm Oil Board (Licensing) Regulations. http://www.mpob.gov.my/en/licensing-a-enforcement/law-a-legislation/511-achievements (2005). Accessed 11 Jan 2017
  36. 36.
    Crabbe, E., Nolasco-Hipolito, C., Kobayashi, G., Sonomoto, K., Ishizaki, A.: Biodiesel production from crude palm oil and evaluation of butanol extraction and fuel properties. Process Biochem. 37, 65–71 (2001)CrossRefGoogle Scholar
  37. 37.
    Tompkins, C., Perkins, E.G.: The evaluation of frying oils with the p-anisidine value. J Am Oil Chem Soc. 76, 945–947 (1999)CrossRefGoogle Scholar
  38. 38.
    Murugan, P., Chhajer, P., Kosugi, A., Arai, T., Brigham, C.J., Sudesh, K.: Production of P(3HB-co-3HHx) with controlled compositions by recombinant Cupriavidus necator Re2058/pCB113 from renewable resources. Clean Soil Air Water. 44, 1234–1241 (2016)Google Scholar
  39. 39.
    Budde, C.F., Riedel, S.L., Hübner, F., Risch, S., Popović, M.K., Rha, C., Sinskey, A.J.: Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium. Appl Microbiol Biotechnol. 89, 1611–1619 (2011)CrossRefGoogle Scholar
  40. 40.
    Coors, E.A., Seybold, H., Merk, H.F., Mahler, V.: Polysorbate 80 in medical products and nonimmunologic anaphylactoid reactions. Ann Allerg Asthma Im. 95, 593–599 (2005)CrossRefGoogle Scholar
  41. 41.
    Ng, K.S., Ooi, W.Y., Goh, L.K., Shenbagarathai, R., Sudesh, K.: Evaluation of jatropha oil to produce poly(3-hydroxybutyrate) by Cupriavidus necator H16. Polym Degrad Stabil. 95, 1365–1369 (2010)CrossRefGoogle Scholar
  42. 42.
    Kahar, P., Tsuge, T., Taguchi, K., Doi, Y.: High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain. Polym Degrad Stabil. 83, 79–86 (2004)CrossRefGoogle Scholar
  43. 43.
    Nomura, C.T., Tanaka, T., Gan, Z., Kuwabara, K., Abe, H., Takase, K., Taguchi, K., Doi, Y.: Effective enhancement of short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production by coexpression of genetically engineered 3-ketoacyl-acyl-carrier-protein synthase III (fabH) and polyhydroxyalkanoate synthesis genes. Biomacromolecules 5, 1457–1464 (2004)CrossRefGoogle Scholar
  44. 44.
    Ling, X., Spruiell, J.E.: Analysis of the complex thermal behavior of poly(L-lactic acid) film. I. Samples crystallized from the glassy state. J Polym Sci Pol Phys. 44, 3200–3214 (2006)CrossRefGoogle Scholar
  45. 45.
    Melero, J.A., Bautista, L.F., Morales, G., Iglesias, J., Sánchez-Vázquez, R.: Biodiesel production from crude palm oil using sulfonic acid-modified mesostructured catalysts. Chem Eng J. 161, 323–331 (2010)CrossRefGoogle Scholar
  46. 46.
    Kek, Y.-K., Lee, W.-H., Sudesh, K.: Efficient bioconversion of palm acid oil and palm kernel acid oil to poly(3-hydroxybutyrate) by Cupriavidus necator. Can J Chem. 86, 533–539 (2008)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Ecobiomaterial Research Laboratory, School of Biological SciencesUniversiti Sains MalaysiaMindenMalaysia

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