Neurotoxicity Research

, Volume 36, Issue 3, pp 441–451 | Cite as

Panax notoginsenoside Rb1 Restores the Neurotrophic Imbalance Following Photothrombotic Stroke in Rats

  • Chun-Yan Yang
  • Jian-Yu Yang
  • Yun-Xia Xiong
  • Xue-Feng Zhuang
  • Hui Su
  • Sheng Hu
  • Jia-Qing Ma
  • Xin-Fu Zhou
  • Hai-Yun LuoEmail author
  • Jun SunEmail author
Original Article


Mature brain-derived neurotrophic factor (mBDNF) has neuroprotection in cerebral ischemia. Conversely, the precursor of brain-derived neurotrophic factor (proBDNF) has the opposite function to its mature form, inducing apoptosis. However, whether the neuroprotection of Panax notoginsenoside Rb1 (PNS-Rb1) on ischemic stroke is due to, at least partially, its modulation of suppressing proBDNF/P75NTR/sortilin or upregulation of mBDNF is not clear. To test this hypothesis, rats induced by photothrombotic stroke were treated with PNS-Rb1 100 mg/kg or nimodipine 1 mg/kg twice a day until 3, 7, and 14 days. Our data indicate that PNS-Rb1 significantly reduced cerebral infarction rate, proBDNF/P75NTR/sortilin, and plasminogen activator inhibitor-1 (PAI-1) protein levels, and improved sensorimotor dysfunctions induced by ischemic stroke, upregulation of BDNF/TrkB levels, and its processing enzymes (tissue plasminogen activator, tPA) in a time-dependent manner. Taken together, our findings indicate that the improvement of sensorimotor dysfunctions by PNS-Rb1 following ischemic stroke is made, at least partially, by activating the BDNF/TrkB and inhibiting proBDNF/sortilin/P75NTR.


Panax notoginsenoside Rb1 Photothrombosis BDNF TrkB proBDNF 


Author Contribution Statements

Study design: HY Luo and J Sun. Study conduct: CY Yang and JY Yang. Data collection: YX Xiong. Data analysis: H Su, S Hu, and XF Zhuang. Data interpretation: JQ Ma. Drafting manuscript: CY Yang and HY Luo. Revising manuscript content: XF Zhou. All authors have read and approved the final submitted manuscript.

Funding Information

This work was supported by the National Natural Science Foundation of China (81760064), Yunnan Applied Basic Research Project(2019FA040), Joint Special Fund Project of Yunnan Provincial Science and Technology Department-Kunming Medical University (2017FE467-025, 160, 163, 2017FE468-135, U012017515, and 2018FE001(-162)), Scientific Research Fund of Yunnan Provincial Education Department (2015Y180), and Innovative Experimental Program for Undergraduates in Kunming Medical University.

Compliance with Ethical Standards

All experimental procedures involving animals were approved by the Animal Ethics Committee of Kunming Medical University and conducted between 7 a.m. and 7 p.m. in accordance with the guidelines of the National Health and Medical Research Council of China. Rats were acclimatized for a week before any procedures were initiated.

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Chun-Yan Yang
    • 1
  • Jian-Yu Yang
    • 1
  • Yun-Xia Xiong
    • 1
  • Xue-Feng Zhuang
    • 1
  • Hui Su
    • 1
  • Sheng Hu
    • 1
  • Jia-Qing Ma
    • 2
  • Xin-Fu Zhou
    • 3
  • Hai-Yun Luo
    • 1
    Email author
  • Jun Sun
    • 4
    Email author
  1. 1.Department of Pharmacology, College of Basic MedicineKunming Medical UniversityKunmingPeople’s Republic of China
  2. 2.Department of Basic Medical Experiment, College of Basic MedicineKunming Medical UniversityKunmingPeople’s Republic of China
  3. 3.School of Pharmacy and Medical Sciences, Sansom InstituteUniversity of South AustraliaAdelaideAustralia
  4. 4.Department of Anatomy, College of Basic MedicineKunming Medical UniversityKunmingPeople’s Republic of China

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