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Antiparasitic effects of Zataria multiflora essential oil nano-emulsion on larval stages of Echinococcus granulosus

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Abstract

Various protoscolicidal agents are indicated for the prevention of recurrence of cystic echinococcosis caused by the larval stage of Echinococcus granulosus; however, most of these chemicals have adverse side effects. This study evaluated the effects of Zataria multiflora essential oil (ZEO) nano-emulsion and emulsion at the concentrations of 1, 2, 5, 10, 15, and 20 µl/ml on E. granulosus protoscoleces. Albendazole (5 mg/ml), normal saline, and nano-emulsion without ZEO served as control groups. Optimal concentrations of ZEO nano-emulsion and emulsion on the microcysts were also investigated. ZEO emulsion at a concentration of 20 µl/ml for the duration of 15 min and nano-emulsion for the duration of 10 min resulted in the death of 100% of the protoscoleces. Additionally, densely packed aggregates were formed inside the microcysts treated with ZEO nano-emulsion and emulsion at a concentration of 20 µl/ml after 40 min, but the complete destruction of laminated layers did not occur. The results indicate that ZEO nano-emulsions have a higher protoscolicidal effect than its emulsion, but these two compounds had similar effects on microcysts.

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Acknowledgements

This study is a result of an approved master’s thesis from Zanjan University of Medical Sciences (A-12-95-12; ZUMS.REC.1396.172). The authors of this article would like to sincerely thank colleagues in the Department of Parasitology and Mycology at Zanjan University of Medical Sciences for their assistance in this project.

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Correspondence to Negin Torabi.

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Karimi Yazdi, M., Haniloo, A., Ghaffari, A. et al. Antiparasitic effects of Zataria multiflora essential oil nano-emulsion on larval stages of Echinococcus granulosus. J Parasit Dis (2020). https://doi.org/10.1007/s12639-020-01193-w

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Keywords

  • Echinococcus granulosus
  • Protoscoleces
  • Microcyst
  • Nano-emulsion
  • Emulsion
  • Zataria multiflora
  • In vitro