Development of membrane-based flow-through assay for detection of trypanosomosis in equines
A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of Trypanosoma specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in < 5 min. The results of the flow-through assay were comparable with the methods like ELISA, dot blot assay and lateral flow assay. The developed assay showed high efficiency for detection of anti-Trypanosoma antibodies in equine sera and can be used for quick screening of serum samples under field conditions.
KeywordsTrypanosomosis Trypanosoma evansi Flow-through assay Gold nanoparticles Rapid immunoassay
This study was supported by Department of Science and Technology (DST), Govt. of India under DST-IDP Project (IDP/MED/2013/09). We are grateful to the Director, National Research Centre on Equines, Hisar for providing necessary facilities to conduct the study.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
The animal experimentation was carried out according to rules and regulations set forth by “Committee for the Purpose of Control and Supervision of Experiments on Animals” (CPCSEA) Animal Welfare Division, Ministry of Environment, and Government of India. The research protocol for experimentation was duly approved by the “Institute Animal Ethics Committee” of the National Research Centre on Equines, Hisar, Haryana, India.
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