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Journal of Parasitic Diseases

, Volume 43, Issue 4, pp 718–725 | Cite as

Assessment of Haemonchus contortus larval and adult somatic antigens in sero-diagnosis of haemonchosis in naturally infected sheep and goats

  • Noha M. F. HassanEmail author
  • Dina Aboelsoued
  • Tarek K. Farag
  • Soad E. Hassan
  • Nadia M. T. Abu El Ezz
Original Article
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Abstract

The current work was carried out to evaluate the potency of larval and adult somatic Haemonchus contortus (H. contortus) antigens in detection of haemonchosis among sheep and goats using ELISA. Two hundred and forty-three fecal and blood samples were randomly collected from small ruminants (107 sheep and 136 goats) in Beni-Suef Governorate, Egypt, during the period from June to August 2018. The fecal analysis exhibited that 26.33% of the small ruminants were infected with gastrointestinal nematodes. The overall prevalence of H. contortus was reached 22.22% whereas it was 27.10% and 18.38% among sheep and goats, respectively. The current study elucidated that the larval antigen has claimed more superior diagnostic results compared to the adult somatic H. contortus antigen. The apparent overall sero-prevalence among small ruminants was reached 51.85%. Separately, it was 64.48% in sheep and 41.91% in goats. The larval antigen had proved 96.55% sensitivity and 47.43% specificity, for sheep serum samples. Meanwhile, sensitivity and specificity for goats’ sera were 100% and 71.17%, respectively. Diagnostic efficacy of ELISA was recorded 60.74% in sheep and 76.47% in goats. This study deduced that the larval antigen has proved the priority and the potency for diagnosis of H. contortus infection. Moreover, haemonchosis is a prevalent disease among the examined sheep and goats.

Keywords

Haemonchus contortus Larval antigen Adult somatic antigen ELISA Sheep and goats 

Notes

Authors’ contributions

NMTAE, NMFH, SEH designed, supervised and provided the steering for the experiment. NMFH and TKF clinically examined the animals and collected the samples. NMFH, TKF and NMTAE examined the fecal samples. NMFH carried out the experimental infection. SEH, NMFH and DA prepared the antigens and applied ELISA. NMFH, SEH, NMTAE, DA and TKF analyzed and discussed the data. NMFH, SEH, DA and NMTAE edited the manuscript. NMFH revised and reviewed the manuscript for publication. All authors read and approved the final article.

Compliance with ethical standards

Conflict of interest

The authors declare that the article has no conflict of interest.

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Copyright information

© Indian Society for Parasitology 2019

Authors and Affiliations

  • Noha M. F. Hassan
    • 1
    Email author
  • Dina Aboelsoued
    • 1
  • Tarek K. Farag
    • 1
  • Soad E. Hassan
    • 1
  • Nadia M. T. Abu El Ezz
    • 1
  1. 1.Department of Parasitology and Animal DiseasesNational Research Centre (NRC)Dokki, GizaEgypt

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