In vitro efficacy of two terpenes against ancyrocephalid monogeneans from Nile tilapia
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Terpenes are naturally produced compounds with a broad range of biological activities. Currently, there is limited information regarding the anthelminthic effect of terpenes against monogenean parasites of fish. The aim of this work was to evaluate the in vitro efficacy of two terpenes [α-terpinene and (+)-limonene oxide] against ancyrocephalid monogeneans found on farmed Nile tilapia (Oreochromis niloticus). (+)-Limonene oxide was more effective in killing these parasites than α-terpinene, with 86 and 90% mortality at concentrations of 36 and 55.4 mg/L, respectively, with a 5-h treatment. The estimated 5-h EC50 of (+)-limonene oxide was 4.8 mg/L. Even though this compound has the potential to be used as an anthelmintic compound in finfish aquaculture, before in vivo experiments are performed, additional studies are needed to find a more effective concentration, as well as to evaluate other terpenic compounds.
KeywordsParasitic Platyhelminthes Control Treatment Essential oils Finfish aquaculture
FNMS, EJFA, study design and manuscript writing; VHCB, DGLM, collection and analyses of data; RMMG, laboratory work; JAMS, contributed reagents, data analyses, and manuscript revision.
This research was funded by the National Council of Science and Technology of Mexico through the Grant FORDECYT 292474.
Compliance with ethical standards
Conflict of interest
The authors declares that they have no conflict of interest.
The authors assert that all procedures contributing to this work comply with the ethical standards of the relevant national and institutional guides on the care and use of laboratory animals.
All data generated or analyzed during this study are available from the corresponding author on reasonable request.
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