Genotyping of Echinococcus granulosus isolates from livestock based on mitochondrial cox1 gene, in the Markazi province, Iran

  • Behnam Abedi
  • Amir Hossein Maghsood
  • Behzad Khansarinejad
  • Mohammad Fallah
  • Mohammad Matini
  • Shirzad Gholami
  • Abdol Sattar Pagheh
  • Reza GhasemikhahEmail author
Original Article


Hydatidosisis a parasitic disease caused by the larval stage of Echinococcus granulosus with different genotypes, and major complications in vital organs such as liver, lungs and, brain. Also, this parasite can infect animals and cause economic damages. Recently, some investigations indicated that the genetic variation of the parasite affects the antigenic, immunogenic and pathogenic features. Therefore, present study conducted to genotyping of the E. granulosus larva based on mitochondrial cox1 gene in livestock in the endemic areas of Markazi province, Iran. In this study, 49 hydatid cysts samples collected from 36 sheep, 11 goats and 2 cattle from different slaughterhouses of Markazi province in central part of Iran, 2017. The mitochondrial cox1 gene was amplified and genotyping were accomplished using sequence analysis. The sequencing analysis indicated that the main genotype G1 (61%) and G3 (37%) were identified. Also, one of the samples shows similarity with the G2 (2%) genotype. The results showed the statistically significant differences between the genotypes in different livestock (P < 0.05). This study indicated that the main genotypes of E. granulosus in Markazi province are G1 and G3 which are related to dog/sheep strain. Therefore, parasite control in dogs and sheep can reduce the risk of transmission of infection to humans.


Echinococcus granulosus cox1 Livestock Iran 



We are very grateful to Dr. Mohammad Amin Tabatabaiefar for her helpful consultation and comments on the manuscript.

Author’s contributions

This study was conducted by BA, AHM, BK, MF, MM, SG, ASP, and RG. Also, RG designed the study. Laboratory evaluation was performed by BA, AHM and BK. Manuscript preparation was conducted by MF, MM, SG, ASP and RG. BA and AHM participated in the data analysis. BA and RG performed the statistical analysis. All authors includes BA, AHM, BK, MF, MM, SG, ASP and RG read and approved the final manuscript.


Vice-chancellor for Research and Technology, Hamadan University of Medical Sciences, Hamadan, Iran (Grant No. 9511267049).

Compliance with ethical standards

Conflict of interest

There was not any conflict of interests by all authors.

Ethical standard

All experimental procedures were approved by the Ethics Committee of Arak University of Medical Sciences, Iran.


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Copyright information

© Indian Society for Parasitology 2019

Authors and Affiliations

  1. 1.Department of Medical Parasitology and MycologyHamadan University of Medical SciencesHamadanIran
  2. 2.Department of Medical MicrobiologyArak University of Medical SciencesArākIran
  3. 3.Department of Parasitology, Toxoplasmosis Research CenterMazandaran University of Medical SciencesSariIran
  4. 4.Department of Medical Parasitology and MycologyArak University of Medical SciencesArākIran

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