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Role of stellate cells in hepatic echinococcosis in cattle

  • Hasan Tarik AtmacaEmail author
  • Aycan Nuriye Gazyagci
  • Osman Safa Terzi
  • Tugçe Sumer
Original Article
  • 9 Downloads

Abstract

Hydatid cysts formed by the metacestodes of Echinococcus granulosus. Cattle suffering from hydatid cyst shows fluid-filled structures, especially in liver. These parasite-induced cysts localized by forming fibrous capsules in the liver. Fibrogenesis is the host immune response in the liver against these parasites. Hepatic stellate cells (HSCs) are localized perisinusoidal space also known as vitamin A-storing cells, characterize the important fibrogenic cell type. In this study, livers from 15 animals with hydatid cyst and 8 healthy animals were used. Hematoxylin and Eosin, masson trichrome staining were performed on the prepared liver sections. Microscopically, cysts were bordered eosinophilic necrotic debris blended with degenerate neutrophils, macrophages, eosinophils, lymphocytes, plasma cells and multinucleated giant cells, which extend into the adjacent fibrous connective tissue. In Masson trichrome staining, the fibrous connective tissue was observed surrounding of hydatid cyst. Glial fibrillary acidic protein (GFAP), collagen I, GFAP/collagen I, positive cells were investigated using either indirect single- or double-labeling immunohistochemical staining. The results indicated that anti-GFAP-positive staining was seen in areas including fibrous tissue just under the foreign body giant cells surrounding the cyst wall. In double immunohistochemical staining, it was observed that HSCs labeled with anti-GFAP antibody in the fibrous connective tissue also labeled anti-collagen I antibody. This study shows that HSCs may responsible for synthesis the collagen I in the development of parasitic fibrosis in cystic echinococcosis in the liver of cattle.

Keywords

Hydatid cyst Echinococcosis Cattle GFAP Collagen I Immunohistochemistry 

Notes

Author contributions

HTA, study conception, analysis of results and manuscript writing. ANG, OST and TS, sample preparation, macroscopic and microscopic analysis, and manuscript writing. All authors have participated actively in the study and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

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Copyright information

© Indian Society for Parasitology 2019

Authors and Affiliations

  1. 1.Department of Pathology, Faculty of Veterinary MedicineBalikesir UniversityBalikesirTurkey
  2. 2.Department of Parasitology, Faculty of Veterinary MedicineKırıkkale UniversityKirikkaleTurkey
  3. 3.Department of Internal Medicine, Faculty of Veterinary MedicineAnkara UniversityAnkaraTurkey
  4. 4.Department of Pathology, Faculty of Veterinary MedicineKırıkkale UniversityKirikkaleTurkey

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