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Helicobacter pylori and enteric parasites co-infection among diarrheic and non-diarrheic Egyptian children: seasonality, estimated risks, and predictive factors

  • Asmaa Ibrahim
  • Yasser B. M. Ali
  • Amal Abdel-Aziz
  • Ayman A. El-Badry
Original Article
  • 29 Downloads

Abstract

Helicobacter pylori (H. pylori) and intestinal parasites are known for their high prevalence in children. Both of them infect the gastrointestinal tract with overlapping clinical pictures. This study was conducted to determine H. pylori prevalence and its association with intestinal parasites in children, moreover to estimate risk and predictive factors for their detection in stool samples. Single fecal samples were collected from 226 Egyptian pediatric patients (125 diarrheic and 101 non-diarrheic) attending gastroenterology outpatients’ clinics, from February 2016 to June 2017. All stool specimens were microscopically examined to search for ova and parasites. Copro-DNAs detection of H. pylori and Cryptosporidium were performed using nested-PCR assays. H. pylori was detected molecularly in 36.8% of the total study population, with a higher prevalence in diarrheic than in non-diarrheic children. Intestinal parasites were detected in 27.4% of the total study populations, of these, 43.9% had co-existence with H. pylori colonized patients and was significantly associated with Cryptosporidium spp. and G. intestinalis. Estimated risk of the presence of H. pylori was in January. Our data provide a better understanding of the epidemiology of H. pylori infection when associated with intestinal parasites. H. pylori co-existence with G. intestinals and Cryptosporidium may suggest the association of H. pylori infection with markers of fecal exposure. Whether H. pylori provides favorable conditions for intestinal parasitosis or vice versa, still further investigations are needed with an emphasis upon determining correlation with gut microbiomes.

Keywords

Helicobacter pylori Intestinal parasites Risk factors Diarrhea Children Egypt 

Notes

Authors contribution

AI: corresponding author, participate in all stages from study design to manuscript writing and revision, YBMA: participate in study design and manuscript revision); AA-A provide technical help; AAE-B: participated in Study design, supervised the lab work, analysis and interpretation of data and involved in drafting the manuscript.

Funding

This research was self-funded and did not receive any grants from any funding agency.

Compliance with ethical standards

Conflict of interest

The authors have declared that no competing interest exists.

Ethical approval

Ethical board of University of Sadat City, Genetic Engineering, and Biotechnology Research Institute, Egypt approved the study. Parents of all the children included in the study were verbally informed about the study’s aims, and collection of the specimens was done after their consent was obtained.

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Copyright information

© Indian Society for Parasitology 2019

Authors and Affiliations

  1. 1.Diagnostic and Research Unit of Parasitic Diseases (DRUP), Department of Medical Parasitology, Kasr Al-Ainy Faculty of MedicineCairo UniversityCairoEgypt
  2. 2.Genetic Engineering and Biotechnology Research InstituteUniversity of Sadat CitySadat CityEgypt
  3. 3.Department of Microbiology, Faculty of MedicineImam Abdulrahman Bin Faisal UniversityDammamSaudi Arabia

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