Journal of Parasitic Diseases

, Volume 43, Issue 1, pp 103–112 | Cite as

In-vitro and in silico efficacy of isolated alkaloid compounds from Rauvolfia tetraphylla L. against bovine filarial parasite Setaria cervi: a drug discovery approach

  • Dipti Ranjan BeheraEmail author
  • Sunita Bhatnagar
Original Article


Bioassay guided isolation from the leaves of Rauvolfia tetraphylla L. resulted in the isolation and characterization of three compounds of alkaloid in nature namely, Curan-17-oic acid (F1); 18, 19-Secoyohimban (F2) and Reserpiline (F3). Macrofilaricidal activity of three compounds was tested against bovine filarial parasite Setaria cervi using in vitro assays and supported by in silico docking analysis on glutathione-S-transferase (GST) enzyme of Wuchereria bancrofti. All the molecules inhibited GST enzyme to some extent 35.78%, 78.22% and 64.21% respectively. Results were supported by molecular docking studies, which showed docking scores for compound F1 (− 5.14), compound F2 (− 7.19) and compound F3 (− 7.2) on GST enzyme. Thus, in conclusion the in vitro and in silico studies indicated that isolated compounds are promising, inexpensive and widely available natural leads, which can be designed and developed into the macrofilaricidal drugs.


Rauvolfia tetraphylla Setaria cervi MTT Glutathione-S-transferase Macrofilaricidal activity In-silico docking 



The authors want to thank the Forest and Environment Dept., Govt. of Odisha for providing funding support in the form of state plan project for smooth completion of research work.

Author’s contributions

DRB contributed in collecting plant samples and parasite, isolated the compounds and performed all in vitro and in silico tests on parasites. SB contributed in data interpretation and critical reading of the manuscript.

Compliance with ethical standards

Conflict of interest

No potential conflict of interest was reported by the authors.


  1. Ahmad R, Srivastava AK (2008) Inhibition of filarial glutathione S-transferase by various classes of compounds and their evaluation as novel antifilarial agents. Helminthologia 45(3):114–120CrossRefGoogle Scholar
  2. Ardelli BF, Guerriero SB, Prichard RK (2005) Genomic organization and effects of ivermectin selection on Onchocerca volvulus P-glycoprotein. Mol Biochem Parasitol 143:58–66CrossRefGoogle Scholar
  3. Azeez S, Babu RO, Aykkal R, Narayanan R (2012) Virtual screening and in vitro assay of potential drug like inhibitors from spices against glutathione-S-transferase of filarial nematodes. J Mol Model 18:151–163CrossRefGoogle Scholar
  4. Baskaran G, Salvamani S, Ahmad SA, Shaharuddin NA, Pattiram PD, Shukor MY (2015) HMG-CoA reductase inhibitory activity and phytocomponent investigation of Basella alba leaf extract as a treatment for hypercholesterolemia. Drug Deg Dev Therapy 9:509–517CrossRefGoogle Scholar
  5. Behera DR, Bhatnagar S (2017) Macrofilaricidal activity of leaf extracts of Rauvolfia tetraphylla L. against bovine filarial parasite Setaria cervi. Int J Pharm Phytochem Res 9(9):1217–1222Google Scholar
  6. Brophy PM, Pritchard DI (1994) Parasitic helminth glutathione S-transferases: an update on their potential as targets for immunoand chemotherapy. Exp Parasitol 79:89–96CrossRefGoogle Scholar
  7. Campbell AM, van Eldik AJ, Liebau E, Barrett J, Brophy PM, Teesdale-Spittle PH, Wang MF (2001) Towards validation of glutathione S-transferase (GST) as a filarial nematode drug target. Chem Biol Interact 133:240–243Google Scholar
  8. Comley JCW, Rees MJ, Turner CH, Jenkins DC (1989) Colorimetric quantitation of filarial viability. Int J Parasitol 19(1):77–83CrossRefGoogle Scholar
  9. Dreyer G, Noroes J, Addiss D (1997) The silent burden of sexual disability associated with lymphatic filariasis. Acta Trop 63(1):57–60CrossRefGoogle Scholar
  10. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139Google Scholar
  11. Henauer SA, Gillespie HK, Hollister LE (1984) Yohimbine and the model anxiety state. J Clin Psychiatry 45:512–515Google Scholar
  12. Hussein HM, Hameed IH, Ibraheem OA (2016) Antimicrobial Activity and spectral chemical analysis of methanolic leaves extract of Adiantum Capillus-Veneris using GC-MS and FTIR spectroscopy. Int J Pharm Phytochem Res 8(3):369–385Google Scholar
  13. Kalani K, Kushwaha V, Sharma P, Verma R, Srivastava M (2014) In Vitro, in silico and in vivo studies of ursolic acid as an anti-filarial agent. PLoS ONE 9(11):e111244CrossRefGoogle Scholar
  14. Kardono LBS, Tsauri S, Padmawinata K, Pezzuto JM, Kinghorn AD (1990) Cytotoxic constituents of the bark of Plumeria rubra collected in Indonesia. J Nat Prod 53(6):1447–1455CrossRefGoogle Scholar
  15. Kaushal NA, Kaushal DC, Ghatak S (1987) Identification of antigenic proteins of Setaria cervi by immunoblotting technique. Immun Invest 16:139–149CrossRefGoogle Scholar
  16. Lakshmi V, Joseph SK, Srivastava S, Verma SK, Sahoo MK et al (2010) Antifilarial activity in vitro and in vivo of some flavonoids tested against Brugia malayi. Acta Trop 116:127–133CrossRefGoogle Scholar
  17. Lazdins J, Kron M (1999) New molecular targets for filariasis drug discovery. Parasitol Today 15(8):305–306CrossRefGoogle Scholar
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–276Google Scholar
  19. Malinowska I, Gadzikowska M, Waksmundzka-Hajnos M (2005) Mobile-phase velocity: a tool for separation of alkaloids by OPLC. J Planar Chromatogr 18:176CrossRefGoogle Scholar
  20. Masoko P, Eloff JN (2007) Screening of twenty-four south african combretum and six Terminalia species (combretaceae) for antioxidant activities. Afr J Tradit CAM 4:231–239Google Scholar
  21. Mathew N, Paily NKP, Vanamail AP, Kalyanasundaram M, Balaraman K (2002) Macrofilaricidal activity of the plant Plumbago indica/rosea in vitro. Drug Dev Res 56:33–39CrossRefGoogle Scholar
  22. Maurya A, Gupta S, Srivastava SK (2013) Large-scale separation of antipsychotic alkaloids from Rauwolfia tetraphylla L. by pH-zone-refining fast centrifugal partition chromatography. J Sep Sci 36:407–413CrossRefGoogle Scholar
  23. Mukherjee M, Misra S, Chatterjee RK (1998) Development of in vitro screening system for assessment of antifilarial activity of compounds. Acta Trop 70:251–255CrossRefGoogle Scholar
  24. Murthy PK (1999) Evaluation of two in vitro systems employing Brugia malayi parasite for prescreening of potential antifilarials. Curr Sci 77(8):1084–1089Google Scholar
  25. Ogunlesi M, Okiei W, Osibote EA (2010) Analysis of the essential oil from the leaves of Sesamum radiatum, a potential medication for male infertility factor, by gas chromatography–mass spectrometry. Afr J Biotech 9(7):1060–1067CrossRefGoogle Scholar
  26. Panwar GS, Guru SK (2011) Alkaloid profiling and estimation of Reserpine in Rauwolfia serpentina plant by TLC, HPTLC and HPLC. Asian J Plant Sci 10(8):393–400CrossRefGoogle Scholar
  27. Poisson J (1959) Research on the alkaloids of Rauwolfia vomitoria Afz. roots (Apocynaceae). Trav Lab Matiere Med Pharm Galenique Fac Pharm Paris 44:2–118Google Scholar
  28. Rajesh A, Shamsudin M (2017) In silico molecular docking studies on phytocompounds from the plant Kalanchoe pinnata targeting the pi-class glutathione-s-transferase of Wuchereria bancrofti. Int J Zool Appl Biol 2(5):258–265Google Scholar
  29. Sahare KN, Singh V (2013) Antifilarial activity of ethyl acetate extract of Vitex negundo leaves in vitro. Asian Pac J Trop Med 6:689–692CrossRefGoogle Scholar
  30. Sahi NM (2016) Evaluation of insecticidal activity of bioactive compounds from Eucalyptus citriodora against Tribolium castaneum. Int J Pharm Phytochem Res 8(8):1256–1270Google Scholar
  31. Schwab AE, Boakye DA, Kyelem D, Prichard RK (2005) Detection of benzimidazole resistance-associated mutations in the filarial nematode Wuchereria bancrofti and evidence for selection by albendazole and ivermectin combination treatment. Am J Trop Med Hyg 73:234–238CrossRefGoogle Scholar
  32. Shanab SMM, Shalaby EA, Lightfoot DA, El-Shemy HA (2010) Allelopathic effects of water hyacinth [Eichhornia crassipes]. Plus One 5:e13200CrossRefGoogle Scholar
  33. Sofowora A (1993) Medicinal plants and traditional medicines in Africa. Spectrum Books, Ibadan, p 150Google Scholar
  34. Srinivasan L, Mathew N, Karunan T, Muthswamy K (2011) Biochemical studies on glutathione S-transferase from the bovine filarial worm Setaria digitata. Parasitol Res 109:213–219CrossRefGoogle Scholar
  35. Srivastava A, Tripathi AK, Pandey R, Verma RK, Gupta MM (2006) Quantitative determination of reserpine, ajmaline and ajmalicine in Rauvolfia serpentina by reversed-phase high-performance liquid chromatography. J Chromatogr Sci 44:557–560CrossRefGoogle Scholar
  36. Trease GE, Evans WC (1989) Pharmacognosy, 13th edn. Bailliere Tindall, London, pp 176–180Google Scholar
  37. Veerapathran A, Dakshinamoorthy G, Gnanasekar M, Reddy MVR, Kalyanasundaram R (2009) Evaluation of Wuchereria bancrofti GST as a vaccine candidate for lymphatic filariasis. PLoS Negl Trop Dis 3(6):e457CrossRefGoogle Scholar
  38. WHO (2009) Global programme to eliminate lymphatic filariasis. Wkly Epidemiol Record 84:437–444Google Scholar

Copyright information

© Indian Society for Parasitology 2018

Authors and Affiliations

  1. 1.Regional Plant Resource CentreNayapalli, BhubaneswarIndia

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